The recommended working concentration for cell lysate application is 1 mM. It's important to note that orthovanadate can be toxic in the blood at a concentration of 0.12 mM. For further information, you can refer to the publication "Biochim Biophys Acta. 2012 Sep;1818(9):2260-70" with the PMID: 22546530. This publication discusses the effects of sodium orthovanadate on human erythrocytes and neuroblastoma cells in vitro. It mentions that the lethal concentration of vanadium in the blood of a patient was calculated to be about 0.12 mM.
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フォーム
liquid
品質水準
メーカー/製品名
Calbiochem®
保管条件
OK to freeze
avoid repeated freeze/thaw cycles
protect from light
色
colorless
保管温度
−70°C
詳細
A depolymerized vanadate prepared from Sodium Orthovanadate (>Cat. No. 567540) that exhibits significant enhanced ability to inhibit endogenous phosphatases in a competitive manner. Preserves the phosphorylation state in cells and cell lysates. Completely reversible with EDTA and dilution. Recommended concentration in lysis buffer ~ 1 mM. Available as 5 ml or as a set of 3 x 1 ml.
生物化学的/生理学的作用
Primary Target
Phosphatases
Phosphatases
Reversible: yes
包装
Packaged under inert gas
警告
Toxicity: Toxic (F)
物理的形状
Supplied as 10 mg/ml in aqueous NaOH, pH 10.0.
再構成
Following initial thaw, aliquot and freeze (-70°C). Aliquots are stable for up to 1 year at -70°C.
その他情報
Gordon, J.A. 1984. Methods. Enzymol.201, 477.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.
Brown, D.J., and Gordon, J.A. 1984. J. Biol. Chem.259, 9580.
法的情報
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
試験成績書(COA)
製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
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