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  • Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts.

Investigating the potential of under-utilised plants from the Asteraceae family as a source of natural antimicrobial and antioxidant extracts.

Food chemistry (2014-05-20)
O Kenny, T J Smyth, D Walsh, C T Kelleher, C M Hewage, N P Brunton
ABSTRACT

Antimicrobial properties of ethanol and water extracts from eight Asteraceae species were investigated against three Gram positive (Staphylococcus aureus, MRSA and Bacillus cereus) and two Gram negative (Escherichia coli and Salmonella typhimurium) bacterial strains. Ethanol extracts from Centaurea scabiosa, Arctium minus, Taraxacum officinale, Centaurea nigra and Cirsium palustre demonstrated antimicrobial activity against strains of S. aureus, MRSA and B. cereus (MIC=187.5-365μg/ml). Ethanol extracts also had higher antioxidant activities and phenolic content demonstrating a link between these compounds and the bioactivity of these extracts. Further investigation into the phenolic content of ethanol extracts using UPLC-MS/MS lead to the identification and quantification of numerous phenolic compounds in all species including; 18 from Cirsium arvense, 16 from Cirsium vulgare, 19 from C. palustre, 15 from C. nigra, 17 from C. scabiosa, 14 from Sonchus asper, 17 from A. minus and 11 from T. officinale.

MATERIALI
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Descrizione del prodotto

Sigma-Aldrich
Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
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Metanolo, suitable for HPLC, ≥99.9%
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Dimetil solfossido, Hybri-Max, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%
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Dimetil solfossido, ACS reagent, ≥99.9%
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Dimetil solfossido, for molecular biology
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Metanolo, ACS reagent, ≥99.8%
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Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%
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Dimetil solfossido, suitable for HPLC, ≥99.7%
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Acetonitrile, HPLC Plus, ≥99.9%
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Dimetil solfossido, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
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Dimetil solfossido, ReagentPlus®, ≥99.5%
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Acido formico, reagent grade, ≥95%
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Metanolo, HPLC Plus, ≥99.9%
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Carbonato di sodio, powder, ≥99.5%, ACS reagent
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Acido formico, ACS reagent, ≥96%
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Ethyl alcohol, Pure, 200 proof, meets USP testing specifications
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Dimetil solfossido, ≥99.5% (GC), suitable for plant cell culture
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Acetonitrile, ACS reagent, ≥99.5%
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Carbonato di sodio, ACS reagent, anhydrous, ≥99.5%, powder or granules
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2,2-Diphenyl-1-picrylhydrazyl
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Ethyl alcohol, Pure, 190 proof, for molecular biology
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Acido (±)-6-idrossi-2,5,7,8-tetrametilcromano-2-carbossilico, 97%
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Acetonitrile, suitable for HPLC, gradient grade, ≥99.9%
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Acido L-ascorbico, powder, suitable for cell culture, γ-irradiated
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Acido L-ascorbico, BioXtra, ≥99.0%, crystalline
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Quercetin, ≥95% (HPLC), solid
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Metanolo, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
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Metanolo, Laboratory Reagent, ≥99.6%
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Carbonato di sodio, ACS reagent (primary standard), anhydrous, 99.95-100.05% dry basis
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Gallic acid, 97.5-102.5% (titration)