Passa al contenuto
Merck
  • An efficient and economical MTT assay for determining the antioxidant activity of plant natural product extracts and pure compounds.

An efficient and economical MTT assay for determining the antioxidant activity of plant natural product extracts and pure compounds.

Journal of natural products (2010-06-23)
Yunbao Liu, Muraleedharan G Nair
ABSTRACT

Antioxidants scavenge free radicals, singlet oxygen, and electrons in cellular redox reactions. The yellow MTT [3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide] is reduced to a purple formazan by mitochondrial enzymes. NADPH is the basis of established in vitro cell viability assays. An antioxidant assay has been developed utilizing the redox reaction between MTT and selected natural product extracts and purified compounds. This simple, fast, and inexpensive MTT antioxidant assay is comparable with the lipid peroxidation inhibitory assay and can be mechanized to achieve high throughput.

MATERIALI
N° Catalogo
Marchio
Descrizione del prodotto

Sigma-Aldrich
Colesterolo, Sigma Grade, ≥99%
Sigma-Aldrich
Acido L-ascorbico, powder, suitable for cell culture, γ-irradiated
Sigma-Aldrich
Acido L-ascorbico, BioXtra, ≥99.0%, crystalline
Sigma-Aldrich
2,6-di-tert-butil-4-metilfenolo, ≥99.0% (GC), powder
Sigma-Aldrich
Acido L-ascorbico, suitable for cell culture, suitable for plant cell culture, ≥98%
Sigma-Aldrich
Acido linoleico, ≥98%
Sigma-Aldrich
Acido caffeico, ≥98.0% (HPLC)
Sigma-Aldrich
Pyrogallol, ≥98% (HPLC)
Sigma-Aldrich
p-Coumaric acid, ≥98.0% (HPLC)
Sigma-Aldrich
Acido 4-idrossibenzoico, ReagentPlus®, ≥99%
Sigma-Aldrich
Octanoic acid, ≥99%
Sigma-Aldrich
Chlorogenic acid, ≥95% (titration)
Sigma-Aldrich
Colesterolo, powder, BioReagent, suitable for cell culture, ≥99%
Sigma-Aldrich
Stearic acid, Grade I, ≥98.5% (capillary GC)
Sigma-Aldrich
Acido L-ascorbico, reagent grade, crystalline
Sigma-Aldrich
2,6-di-tert-butil-4-metilfenolo, ≥99%, FCC, FG
Sigma-Aldrich
Acido 4-idrossibenzoico, ReagentPlus®, 99%
Sigma-Aldrich
β-Carotene, synthetic, ≥95% (HPLC), crystalline
Sigma-Aldrich
β-Carotene, synthetic, ≥93% (UV), powder
Sigma-Aldrich
Lauric acid, ≥98%, FCC, FG
Sigma-Aldrich
Acido linoleico, liquid, BioReagent, suitable for cell culture
Sigma-Aldrich
Pyrogallol, ACS reagent, ≥99%
Sigma-Aldrich
trans-Ferulic acid, 99%
Sigma-Aldrich
(−)-Epicatechin, ≥90% (HPLC)
Sigma-Aldrich
Decanoic acid, ≥99.5%, FCC, FG
Sigma-Aldrich
Colesterolo, from sheep wool, ≥92.5% (GC), powder
Supelco
Acido L-ascorbico, analytical standard
Sigma-Aldrich
tert-Butylhydroquinone, 97%
Sigma-Aldrich
Camphor, 96%
Sigma-Aldrich
Decanoic acid, ≥98.0%