QR0200

Quantitative RT-PCR ReadyMix^™

One step RT-qPCR for probe-based methods, MMLV & hot-start Taq

• Sinonimo/i: 1-step RT-qPCR mix, Quantitative real-time PCR master mix
• Codice UNSPSC: 41106300
• NACRES: NA.55

Proprietà

• impiego: sufficient for 250 reactions
• Caratteristiche: dNTPs included; hotstart
• tecniche: RT-qPCR: suitable
• Colore: colorless
• input: purified RNA
• Compatibilità: ABI 5700; ABI 7000; ABI 7300; ABI 7500 Fast; ABI 7500; ABI 7700; ABI 7900 HT ; ABI 7900 HT Fast; ABI 7900; ABI StepOne; ABI StepOnePlus; ABI ViiA 7; Bio-Rad CFX384; Bio-Rad CFX96; Bio-Rad MJ Chromo4; Bio-Rad MJ Opticon 2; Bio-Rad MJ Opticon; Bio-Rad MiniOpticon; Bio-Rad MyiQ; Bio-Rad iCycler iQ; Bio-Rad iQ5; Cepheid SmartCycler; Eppendorf^® Mastercycler ep realplex2 s; Eppendorf^® Mastercycler ep realplex; Illumina Eco qPCR; Qiagen Corbett Rotor-Gene 3000; Qiagen Corbett Rotor-Gene 6000; Qiagen Corbett Rotor-Gene Q; Roche LightCycler 480; Strategene Mx3000P; Strategene Mx3005P; Strategene Mx4000
• Metodo di rivelazione: probe-based
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Quantitative RT-PCR ReadyMix^™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart^™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart^™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart^™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods.

Applicazioni

Quantitative RT-PCR ReadyMix^™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR).

Caratteristiche e vantaggi

• The master mix allows consistency and reproducibility from one reaction to the next
• Reduced risk of contamination from multiple pipetting steps
• Reduced set-up time as compared to manual or wax Hot Start methods
• JumpStart^™Taq Polymerase reduces primer-dimer and non-specific product formation
• Broad instrument compatibility
• Includes a separate ROX reference dye vial for reaction normalization

Confezionamento

1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each.

Note legali

This product is for research use only.

Eppendorf is a registered trademark of Eppendorf AG

JumpStart is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Solo come componenti del kit

• Probe Based qRT-PCR ReadyMix 2 X
• Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U

I componenti del kit sono disponibili anche separatamente

• P2192 10X PCR Buffer, Optimized for routine PCR with MgCl₂ included 1.5 mL/vial
• M8787 Magnesium chloride solution, PCR Reagent, 25 mM MgCI₂ solution for PCR 25 mM
• P2192 10X PCR Buffer, Optimized for routine PCR with MgCl₂ included 100 X

Informazioni sulla sicurezza

• Pittogrammi: GHS07,GHS09
• Avvertenze: Warning
• Indicazioni di pericolo: H315,H317,H319,H410
• Consigli di prudenza: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338
• Classi di pericolo: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable