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PKH67GL

Sigma-Aldrich

Kit linker cellulare PKH67 fluorescente verde per la marcatura generale della membrana cellulare

Distributed for Phanos Technologies

Sinonimo/i:

Green PKH membrane labeling kit

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1 KIT
1.750,00 €

1.750,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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Cambia visualizzazione
1 KIT
1.750,00 €

About This Item

Codice UNSPSC:
12352207
NACRES:
NA.32

1.750,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Livello qualitativo

200

Confezionamento

pkg of 1 kit

Condizioni di stoccaggio

protect from light

Fluorescenza

λex 490 nm; λem 502 nm (PKH67 dye)

Metodo di rivelazione

fluorometric

Condizioni di spedizione

ambient

Temperatura di conservazione

room temp

Categorie correlate

Applicazioni

Il kit PKH67 Green Fluorescent Cell Linker per la marcatura aspecifica della membrana cellulare è stato utilizzato:
  • per marcare e quindi studiare gli esosomi espulsi dalle cellule nel cancro della mammella triplo negativo[1]
  • per marcare le cellule apoptotiche[2] al fine di studiare il ruolo del recettore ανβ5 nel legame e nell′internalizzazione delle cellule apoptotiche[3]
  • nell′imaging in fluorescenza.[4]

Il kit serve alla marcatura generale delle membrane cellulari. Il PKH67 ha una coda di carbonio alifatica più lunga del PKH1 e del PKH2, gli altri due coloranti verdi precedentemente descritti per il tracking cellulare in vitro e in vivo. Sulla base della maggiore lunghezza della coda, gli studi in-house hanno mostrato costantemente un trasferimento cellula-cellula ridotto per il PKH67 rispetto al PKH2.
È stata osservata una lenta perdita di fluorescenza negli studi condotti in vivo con PKH1 e PKH2. Il PKH67 può mostrare proprietà simili dal momento che questo comportamento sembra essere caratteristico dei coloranti linker cellulari verdi ma non di quelli rossi. La correlazione tra la ritenzione della membrana cellulare in vitro e l′emivita della fluorescenza in vivo nelle cellule che non si dividono prevede un′emivita della fluorescenza in vivo di 10-12 giorni per il PKH67. Altri coloranti linker cellulari con emivite analoghe sono stati utilizzati per monitorare il traffico di linfociti e macrofagi in vivo nell′arco di 1-2 mesi. Questo suggerisce che il PKH67 potrebbe essere utile anche per gli studi di tracking in vivo di durata moderata.

Linkage

Per ulteriori dettagli tecnici sui coloranti linker cellulari fluorescenti PKH e CellVue®, inclusa un′ampia bibliografia, fare clic qui.

Note legali

CellVue is a registered trademark of Phanos Technologies

Solo come componenti del kit

N° Catalogo
Descrizione
  • Diluent C 6 x 10
  • PKH67 Cell Linker in ethanol .5 mL

Pittogrammi

FlameExclamation mark
GHS02,GHS07

Avvertenze

Danger

Indicazioni di pericolo

H225,H319

Classi di pericolo

Eye Irrit. 2 - Flam. Liq. 2

Codice della classe di stoccaggio

3 - Flammable liquids

Punto d’infiammabilità (°F)

57.2 °F - closed cup

Punto d’infiammabilità (°C)

14 °C - closed cup

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
MKCV4102
MKCQ9079
MKCP3216
MKCQ0194
MKCK9600

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Anjali P Kusumbe et al.
Stem cells (Dayton, Ohio), 27(3), 498-508 (2009-03-04)
Recruitment and localization of endothelial precursors within tumors is a potential area for the development of therapeutics, because their functional contribution to tumor vasculature is realized to be important for cancer cell survival. However, the exact nature of the recruited
alpha v beta 5 integrin recruits the CrkII-Dock180-Rac1 complex for phagocytosis of apoptotic cells
Albert, Matthew L and Kim, Jong-Ii and Birge, Raymond B
Nature Cell Biology, 2(12), 899-899 (2000)
Exosomes from triple-negative breast cancer cells can transfer phenotypic traits representing their cells of origin to secondary cells
O Brien K, et al.
European Journal of Cancer, 49(8), 1845-1859 (2013)
Increased expression of inducible HSP70 in apoptotic cells is correlated with their efficacy for antitumor vaccine therapy
Masse D, et al.
International Journal of Cancer. Journal International Du Cancer, 111(4), 575-583 (2004)
Nobuyoshi Kosaka et al.
The Journal of biological chemistry, 287(2), 1397-1405 (2011-11-30)
Normal epithelial cells regulate the secretion of autocrine and paracrine factors that prevent aberrant growth of neighboring cells, leading to healthy development and normal metabolism. One reason for tumor initiation is considered to be a failure of this homeostatic cell
Visualizza tutti i documenti correlati

Articoli

PKH Linker Kits for Fluorescent Cell Labeling

PKH dyes are easy to use and achieve stable, uniform, and reproducible fluorescent labeling of live cells. PKH dyes are non-toxic membrane stains which produce high signal to noise ratio.

Lipophilic Dye Kits for Cell Tracking

Lipophilic cell tracking dyes enable cancer biologists to track tumor and immune cell functions both in vitro and in vivo. Read the article to choose a right membrane dye kit for cell tracking and proliferation monitoring.

Cell Tracking with Lipophilic Membrane Dyes

Optimal staining is a key component for studying tumorigenesis and progression. Learn useful tips and techniques for dye applications, including examples from recent studies.

PKH and CellVue® Claret Fluorescent Cell Linker Dyes

PKH and CellVue® Fluorescent Cell Linker Kits provide fluorescent labeling of live cells over an extended period of time, with no apparent toxic effects.

Domande

1–10 di 12 domande  

  1. I mark mitochondria for injection with PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling. I want to use another dye to mark another type of mitochondria and inject them together in the same fish egg. Is PKH67 ok for this like PKH26?

    1 risposta

    1. Yes, Product PKH67 would be suitable for this application. In this paper, both Products PKH26 and PKH67 are used in a similar application:
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5679712/

      Utile?

  2. What can be the alternative for removing excess dye after labeling of exosomes using PKH67 dye apart from ultracentrifugation?

    1 risposta

    1. Excess dye is bound to serum or albumin which is added to the cell/dye mixture as a stop reaction. The recommended clean up or dye removal is by standard centrifugation (400 x g for 10 minutes, wash, and repeat). See the Product Information Sheet, page 3, bullet point 8 for more information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/984/984/pkh67glbul.pdf

      Alternate methods for excess dye removal have not been investigated.

      Utile?

  3. Can we remove the excess PKH67 DYE used for the labeling of exosomes using exosome spin column rather than ultracentrifugation.

    1 risposta

    1. The recommended exosome labeling protocol has been optimized to assure the best possible results. The use of exosome spin columns with the PKH and CellVue products has not been validated. See below for a link to our exosome labeling protocol. A spin column method may not offer the best results.

      https://www.sigmaaldrich.com/technical-documents/protocol/cell-culture-and-cell-culture-analysis/imaging-analysis-and-live-cell-imaging/exosome-labeling-pkh

      Utile?

  4. Will Product PKH67, Green Fluorescent Cell Linker Kit, stain dead cells?

    1 risposta

    1. As long as the cell has an intact membrane, the PKH76 dye can label the cell.

      Utile?

  5. What is the difference between Green Fluorescent Cell Linker Kits PKH2 and PKH67?

    1 risposta

    1. PKH2 was one of the early PKH dyes.  The PKH67 dye has a longer aliphatic tail.  There is reduced cell-celldye transfer for PKH67 as compared with PKH2.

      Utile?

  6. What method of fixation can be used for tissue/cells with the PKH Fluorescent Cell Linker Kit for General Cell Membrane Labeling dyes?

    1 risposta

    1. A protocol for visualization of tissue sections can be found in the technical bulletin.  For visualization of stained cells by immunofluorescence or flow cytometry, the cells can be fixed in 2% paraformaldehyde for 15 minutes. The use of other organic solvents will extract the dye from the cells.  If internal labeling is desired, the cells can be permeabilized with saponin (50-75 μg/mL). Here is the link to the Troubleshooting Guide.

      Utile?

  7. What is the Department of Transportation shipping information for this product?

    1 risposta

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

  8. What is the excitation and emission spectra for Product PKH67GL, Green Fluorescent Cell Linker Kit?

    1 risposta

    1. The spectra can be found on the product insert.The product has a maximum excitation at 490 nm and maximum emission at 502 nm.

      Utile?

  9. How many cells can be stained with Product PKH67GL, Green Fluorescent Cell Linker Kit?

    1 risposta

    1. This kit can stain 50 × 107 cells if used as directed (1 × 107 cells stained with 2 × 10-6 M PKH67).

      Utile?

  10. When using Product PKH67GL, PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling, how long will the cells remain stained after treatment?

    1 risposta

    1. Labeled cells that have been washed can be visualized in culture up to 100 days after staining (for non-dividing cells). The dye itself is stable and will divide equally when the cells divide. After staining with PKH dyes, you can observe as many as 8 divisions depending on how brightly the cells were stained initially and the amount of surface area on the cells. Most commonly, 4-6 divisions can be visualized.

      Utile?

1–10 di 12 domande  

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