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P5368

Sigma-Aldrich

Tampone fosfato salino

powder, pH 7.4, BioPerformance Certified, for molecular biology

Sinonimo/i:

PBS

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10 PKG
116,00 €

116,00 €


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10 PKG
116,00 €

About This Item

Numero MDL:
Codice UNSPSC:
12161700
eCl@ss:
32129211
NACRES:
NA.25

116,00 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Richiedi un ordine bulk

Nome del prodotto

Tampone fosfato salino, BioPerformance Certified, pH 7.4

Grado

BioPerformance Certified
for molecular biology

Livello qualitativo

Stato

powder

Condizioni di stoccaggio

(Keep container tightly closed in a dry and well-ventilated place.)

tecniche

immunofluorescence: suitable

Impurezze

BioBurden, tested
endotoxin, tested

Colore

white

pH

7.4

Solubilità

water: 9.07-10.0 g/L, clear, colorless

Cationi in tracce

Fe: ≤10 ppm
heavy metals (as Pb): ≤5 ppm

Compatibilità

suitable for SDS-PAGE
suitable for molecular biology

applicazioni

diagnostic assay manufacturing
histology
sample preparation

Attività estranea

DNase, RNase, Protease, none detected

Stringa SMILE

OP(=O)(O)[O-].OP(=O)([O-])[O-].[Na+].[Na+].[Na+].[Cl-].[Cl-].[K+].[K+]

InChI

InChI=1S/2ClH.2K.3Na.2H3O4P/c;;;;;;;2*1-5(2,3)4/h2*1H;;;;;;2*(H3,1,2,3,4)/q;;5*+1;;/p-5
LOKCTEFSRHRXRJ-UHFFFAOYSA-I

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Applicazioni


  • Rapid and zero-cost DNA extraction from soft-bodied insects for routine PCR-based applications.: This study used phosphate buffered saline (PBS) to develop a cost-effective and efficient method for DNA extraction from insects, facilitating routine PCR-based applications in molecular biology (Jangra & Ghosh, 2022).

  • Injectable amnion hydrogel-mediated delivery of adipose-derived stem cells for osteoarthritis treatment.: PBS was utilized to prepare and deliver adipose-derived stem cells in an injectable hydrogel, showing potential for innovative osteoarthritis treatments (Bhattacharjee et al., 2022).

  • Rapid and sensitive detection of SARS-CoV-2 infection using quantitative peptide enrichment LC-MS analysis.: This research employed PBS for sample preparation in a novel LC-MS method for detecting SARS-CoV-2, providing a rapid and sensitive diagnostic tool (Hober et al., 2021).

Confezionamento

Foil Pouch

Ricostituzione

Contents of one pouch, when dissolved in one liter of distilled or deionized water, will yield 0.01 M phosphate buffered saline (NaCl 0.138 M; KCl - 0.0027 M); pH 7.4, at 25 °C.

Altre note

For additional information on our range of Biochemicals, please complete this form.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Certificati d'analisi (COA)

Lot/Batch Number

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Petra J Roubos-van den Hil et al.
Veterinary microbiology, 202, 58-63 (2016-09-27)
Enterotoxigenic E. coli (ETEC), causing post-weaning diarrhoea, is a major problem in weaned piglets. Individual animal responses to ETEC infection show high variability in animal experiments. Two studies were designed to optimize the ETEC F4ac infection model in piglets by
Jinjin Sun et al.
Nanoscale research letters, 14(1), 248-248 (2019-07-26)
Herein, a magnetic graphene field-effect transistor biosensor was prepared through the transfer of a chemical vapor deposition graphene film onto a glass substrate to produce a sensing film and conductive channel. By fixing 1-pyrenebutanoic acid succinimidyl ester onto graphene film
Jesús Gilberto Arámburo-Galvez et al.
Nutrients, 10(7) (2018-07-18)
Most food allergy cases are associated with a limited group of allergens. This could be attributed to an increased ability of some foods to sensitize and trigger allergic reactions. However, there are no validated animal models to evaluate the sensitizing
Gary Burness et al.
Biology open, 7(4) (2018-04-06)
Female birds transfer maternally derived antibodies (matAb) to their nestlings, via the egg yolk. These antibodies are thought to provide passive protection, and allow nestlings to avoid the costs associated with mounting an innate immune response. To test whether there
Yixi Chen et al.
The European journal of neuroscience, 49(4), 510-524 (2018-11-26)
An emerging treatment for Parkinson's disease (PD) is cell replacement therapy. Authentic midbrain dopaminergic (mDA) neuronal precursors can be differentiated from human embryonic stem cells (hESCs) and human induced pluripotent stem cells (iPSCs). These laboratory-generated mDA cells have been demonstrated

Articoli

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The application note describes a comparative analysis of plasma protein binding results for free/bound drugs using two different sample extraction steps, with and without agitation.

Learn about Supel™ BioSPME C18 high-throughput devices to determine plasma protein binding and decrease the sample preparation time to less than an hour.

Comparative analysis of Supel™ BioSPME 96-Pin device with a rapid equilibrium dialysis technique for accuracy of measured values, sample cleanliness, and workflow time in drug protein binding.

Protocolli

Preparation for biodegradable nanoparticles and their use in transfection protocols .

Domande

1–5 di 5 domande  
  1. What is the shelf-life of product P5368, phosphate buffered saline (PBS)?

    1 risposta
    1. This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.

      Utile?

  2. What is the Department of Transportation shipping information for this product?

    1 risposta
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Utile?

  3. Does Product P5368, Phosphate buffered saline (PBS), contain calcium and magnesium?

    1 risposta
    1. No.  The complete formulation is shown in the product listing.  When reconstituted, the resulting solution is 10 mM phosphate buffer, with 138 mM sodium chloride and 2.7 mM potassium chloride.

      Utile?

  4. What volume of Product P5368, Phosphate buffered saline (PBS), does each pack make?

    1 risposta
    1. When the contents of a pouch is dissolved in 1 Liter of water, it makes 1X PBS, pH 7.4 at 25°C.

      Utile?

  5. If reconstituted with sterile water, will Product P5368, Phosphate buffered saline (PBS), be sterile?

    1 risposta
    1. No.  To make this PBS sterile, the solution should be either sterile-filtered or autoclaved.

      Utile?

Recensioni

Nessuna valutazione

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