![Guanosine 5′-[β,γ-imido]triphosphate trisodium salt hydrate ≥85% (HPLC), powder](/deepweb/assets/sigmaaldrich/product/structures/204/494/05808804-1ca7-44bf-b6c5-d4934dc7cb85/640/05808804-1ca7-44bf-b6c5-d4934dc7cb85.png)
To collect the agarose beads, a centrifugation speed of 1000 x g for 5 minutes should be sufficient, using the A2220 batch method as a guide
P0762
Phosphatase, Alkaline−Agarose from calf intestine
ammonium sulfate suspension
Sinonimo/i:
Alkaline phosphatase-Agarose
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Scegli un formato
50 UNITS
258,00 €
100 UNITS
445,00 €
250 UNITS
954,00 €
1000 UNITS
2.980,00 €
258,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Scegli un formato
Cambia visualizzazione
50 UNITS
258,00 €
100 UNITS
445,00 €
250 UNITS
954,00 €
1000 UNITS
2.980,00 €
About This Item
258,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Origine biologica
bovine (calf) intestine
Stato
ammonium sulfate suspension
Attività specifica
250-1000 units/mL packed gel
Matrice
beaded agarose
Temperatura di conservazione
2-8°C
Categorie correlate
Descrizione generale
Alkaline phosphatase from calf intestine is a glycoprotein with a molecular weight of 140,000. [1]
Applicazioni
Alkaline phosphatase from calf intestine, CIP, removes phosphate groups from the 5′ ends of DNA which aids in cloning techniques to keep DNA from self-ligation. It can be inhibited by homoarginine. [2]
Calf intestinal alkaline phosphatase (CIAP) has been used in a study with the spectrophotometric method to assess the inhibition effect of dansyl-L-phenylalanine. [3] It has also been shown that (CIAP) is able to detoxify the lipopolysaccharide (LPS)-mediated inflammatory response, which could be important in the treatment of LPS-mediated diseases. [4]
Azioni biochim/fisiol
Sigma insoluble enzymes are produced by reacting the conventional "soluble" enzyme with an inert base that results in an insoluble compound retaining the activity of the original enzyme. Applications are almost unlimited. By selecting the "inert" base carefully, a highly active "resin" or "gel" can be produced with which the enzyme reaction can be quickly catalyzed by momentary contact with the substrate in a suitable medium. This can be carried out either in a batch slurry or even through a small column. In either case, the insoluble enzyme does not remain as a contaminant of the reaction mix. It can be readily filtered or centrifuged out of the batch slurry, or remain behind in the column. Many unstable enzymes seem to be much more stable in the insoluble form.
Confezionamento
Package size based on DEA units
Definizione di unità
One unit will hydrolyze 1.0 mmole of p-nitrophenyl phosphate per min at pH9.8 at 37 °C.
Stato fisico
Suspension in 2.0 M (NH4)2SO4, 1 mM MgCl2, 0.1 mM ZnCl2, pH 7
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Elenchi normativi
Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.
EU REACH Annex XVII (Restriction List)
CAS No.
Scegli una delle versioni più recenti:
Certificati d'analisi (COA)
Lot/Batch Number
Non trovi la versione di tuo interesse?
Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
A Bakker et al.
Journal of virology, 70(8), 5511-5518 (1996-08-01)
The Rex protein is an essential regulator of RNA expression in human T-cell leukemia virus types 1 and 2 (HTLV-1 and HTLV-2) that promotes the accumulation of full-length and partially spliced viral transcripts in the cytoplasm. Rex-mediated regulation correlates with
Li-Na Li et al.
Guang pu xue yu guang pu fen xi = Guang pu, 29(10), 2820-2823 (2009-12-30)
To evaluate the inhibition effect of dansyl-L-phenylalanine on calf intestinal alkaline phosphatase (CIAP), UV-Vis spectrophotometric method was employed. It was found that dansyl-L-phenylalanine can selectively inhibit CIAP. The kinetic inhibition processes of dansyl-L-phenylalanine and L-phenylalanine were comparatively studied. The authors'
J E Coleman
Annual review of biophysics and biomolecular structure, 21, 441-483 (1992-01-01)
Alkaline phosphatase was the first zinc enzyme to be discovered in which three closely spaced metal ions (two Zn ions and one Mg ion) are present at the active center. Zn ions at all three sites also produce a maximally
Calf intestinal alkaline phosphatase, a novel therapeutic drug for lipopolysaccharide (LPS)-mediated diseases, attenuates LPS toxicity in mice and piglets
Beumer, C.
Journal of Pharmacology and Experimental Therapeutics, 397, 737-744 (2003)
Rebika Shrestha et al.
iScience, 25(1), 103608-103608 (2022-02-03)
Understanding the spatiotemporal distribution and dynamics of RAS on the plasma membrane (PM) is the key for elucidating the molecular mechanisms of the RAS signaling pathway. Single particle tracking (SPT) experiments show that in cells, KRAS diffuses in at least
-
1. What is the recommended speed for spinning down the phosphatase, alkaline-agarose when using this product?
1 risposta-
Utile?
-
Filtri attivi
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.
