N2635
Nucleoside 5′-Diphosphate Kinase from bovine liver
buffered aqueous glycerol solution, ≥1,000 units/mg protein (biuret)
Sinonimo/i:
NDK, UDPkinase, nonmetastatic23(NM23), uridine diphosphate kinase, ATP:nucleoside diphosphate phosphotransferase, NDPK
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Prodotti consigliati
Origine biologica
bovine liver
Forma fisica
buffered aqueous glycerol solution
Attività specifica
≥1,000 units/mg protein (biuret)
Condizioni di stoccaggio
(Tightly closed)
tecniche
activity assay: suitable
Attività estranea
lactic dehydrogenase, myokinase, β-NADH oxidase, nucleoside monophosphokinase and ATPase ≤0.1%
Temperatura di conservazione
2-8°C
Descrizione generale
Research area: Cell Signaling
Nucleoside 5′-Diphosphate Kinase (NDK) is a ubiquitous housekeeping enzyme. Nucleoside Diphosphate Kinase exists in two isoforms in eukaryotic cells, NDK-A and NDK-B. These enzymes are found expressed both in the mitochondria and the cytoplasm.
Nucleoside 5′-Diphosphate Kinase (NDK) is a ubiquitous housekeeping enzyme. Nucleoside Diphosphate Kinase exists in two isoforms in eukaryotic cells, NDK-A and NDK-B. These enzymes are found expressed both in the mitochondria and the cytoplasm.
Applicazioni
Nucleoside 5′-Diphosphate Kinase has been used:
- nucleoside diphosphate kinase (NDPK) assays
- coupled NDPK-luciferase assay to determine the amounts of Ras-bound guanosine triphosphate (GTP)
- non-metastatic protein 23 (NM23) growth stimulation assay
- in a study to assess inhibition of type I Fc epsilon receptor mediated Ca2+ influx and mediator secretion in rat mucosal mast cells
- in a study to investigate protein synthesis in rabbit reticulocytes
Azioni biochim/fisiol
Nucleoside 5′-Diphosphate Kinases (NDKs) aid in the synthesis of nucleoside triphosphates (NTPs) by transferring a phosphate group from ATP to nucleoside diphosphates (NDPs). NDKs provide NTPs for nucleic acid synthesis, cytidine 5′-triphosphate (CTP) for lipid synthesis, uridine 5′-triphosphate (UTP) for polysaccharide synthesis, and guanosine triphosphate (GTP) for protein elongation, signal transduction, and microtubule polymerization.
Definizione di unità
One unit will convert 1.0 μmole each of TDP and ATP to TTP and ADP per min at pH 7.6 at 25 °C in a coupled system with PK/LDH.
Anticorpo
Enzima
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Prodotti correlati
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Substrato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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I clienti hanno visto anche
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In textbooks of biochemistry, nucleoside diphosphate conversion to a triphosphate by nucleoside diphosphate 'kinases' (NDPKs, also named NME or NM23 proteins) merits a few lines of text. Yet this essential metabolic function, mediated by a multimeric phosphotransferase protein, has effects
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The Biochemical journal, 393(Pt 1), 235-243 (2005-09-15)
In growth-factor-stimulated signal transduction, cell-surface receptors recruit PI3Ks (phosphoinositide 3-kinases) and Ras-specific GEFs (guanine nucleotide-exchange factors) to the plasma membrane, where they produce 3'-phosphorylated phosphoinositide lipids and Ras-GTP respectively. As a direct example of pathway networking, Ras-GTP also recruits and
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