Passa al contenuto
Merck
Tutte le immagini(2)

Documenti fondamentali

Visualizza tutta la documentazione

MAK190

Sigma-Aldrich

ATP Assay Kit

Sufficient for 100 Colorimetric or Fluorometric tests

Sinonimo/i:

Adenosine triphosphate Assay Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(2)

About This Item

Numero CE:
213-579-1
Codice UNSPSC:
12161503
NACRES:
NA.84

Metodo di rivelazione

colorimetric
fluorometric

Malattie correlate

cancer; neurological disorders

Temperatura di conservazione

−20°C

Descrizione generale

Adenosine triphosphate (ATP) is a nucleoside triphosphate formed exclusively in the mitochondria. It is a high-energy molecule described as the energy currency in all living systems. The chemical energy contained in the phosphate bond of ATP drives most cellular processes.[1][2] Genetic disorders such as Leber hereditary optic neuropathy,[2] Leigh syndrome, neuropathy, ataxia, and retinitis pigmentosa [3] affect the generation of ATP in the mitochondria.

Applicazioni

ATP Assay Kit has been used to quantify the ATP concentration by fluorometric detection of ATP in samples.[4]

Compatibilità

Suitable for the determination of ATP in samples such as animal tissue and cell culture samples.

Principio

The ATP Colorimetric/Fluorometric Assay Kit is a sensitive assay for determining ATP in various samples (ranging from 2–10 nmole/well for the colorimetric assay and from 20–1,000 pmole/well for the fluorometric assay). ATP concentration is determined by phosphorylating glycerol, resulting in a colorimetric (570 nm) or fluorometric (lex = 535/lem = 587 nm) product proportional to the amount of ATP present.

Altre note

The fluorometric assay is 10-100 times more sensitive than the colorimetric assay.

Solo come componenti del kit

N° Catalogo
Descrizione
  • ATP Assay Buffer
  • ATP Probe, in DMSO
  • ATP Converter
  • Developer Mix
  • ATP Standard, 1.0 μmole

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

188.6 °F

Punto d’infiammabilità (°C)

87 °C

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
8L09K03540
8E04K03540
8A07K03540
7H23K03540
7D05K03540

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Cerca un COA

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 2

1 of 2

Universal Fluorometric Kinase Assay Kit sufficient for 250 fluorometric tests

Sigma-Aldrich

MAK173

Universal Fluorometric Kinase Assay Kit

HY-LiTE® ATP Standard Solution Positive Control (PC) standard solution (1.0 ng/mL) for performance verification of HY-LiTE® 2/2A instruments, pkg of 5 vials (liquid standard solution)

Millipore

1.30195

HY-LiTE® ATP Standard Solution

E Alirol et al.
Oncogene, 25(34), 4706-4716 (2006-08-08)
Mitochondria are key players in several cellular functions including growth, division, energy metabolism, and apoptosis. The mitochondrial network undergoes constant remodelling and these morphological changes are of direct relevance for the role of this organelle in cell physiology. Mitochondrial dysfunction
Brock A Humphries et al.
Breast cancer research : BCR, 22(1), 60-60 (2020-06-07)
Mitochondrial dynamics underlies malignant transformation, cancer progression, and response to treatment. Current research presents conflicting evidence for functions of mitochondrial fission and fusion in tumor progression. Here, we investigated how mitochondrial fission and fusion states regulate underlying processes of cancer
Sandra Aurora Niño et al.
ACS chemical neuroscience, 10(1), 323-336 (2018-08-25)
Worldwide, every year there is an increase in the number of people exposed to inorganic arsenic (iAs) via drinking water. Human populations present impaired cognitive function as a result of prenatal and childhood iAs exposure, while studies in animal models
Cost of surviving sepsis: A novel model of recovery from sepsis in Drosophila melanogaster.
Kaynar A M, et al.
Intensive care medicine experimental, 4(1), 4-4 (2016)
Rosalba Carrozzo et al.
Bioscience reports, 27(1-3), 105-112 (2007-05-09)
Mitochondrial disorders encompass any medical specialty and affect patients at any age. Likewise, the spectrum of clinical and genetic signatures of these disorders is ample, making a precise diagnosis difficult. We will report some of the major clinical phenotypes observed
Visualizza tutti i documenti correlati

Domande

1–5 di 5 domande  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 risposta

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

      Utile?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 risposta

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Utile?

  3. What is the chemical composition of the reagents?

    1 risposta

    1. The chemical composition of these reagents is proprietary.

      Utile?

  4. Dear sir, We are interested in quantification of ATP levels "ATP Assay Kit" Cat.No:MAK190. Protocol recommends that cells should be deproteinized using 10kDa MWCO spin filter, but no protocol is mentioned. Can you Please send us protocol for the same.

    1 risposta

    1. The Datasheet can be accessed in the DOCUMENTATION section under 'More Documents'. Please see the Datasheet below:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/439/mak190bul.pdf

      Utile?

  5. What is the chemical composition of the reagents?

    1 risposta

    1. The chemical composition of these reagents are proprietary. Please see the link below to review the Product datasheet: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/262/439/mak190bul.pdf

      Utile?

Recensioni

Nessuna valutazione

Filtri attivi

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.