Modification of the original MEM formulation. Contains a succinate buffering system. See formulations table for specific information. Medium may be autoclaved.
Altre note
Modified for autoclaving.
Quantità
Formulated to contain 9.4 grams of powder per liter of medium.
Ricostituzione
Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate.
The Journal of cell biology, 190(2), 197-207 (2010-07-28)
The signaling cascade initiated in response to DNA double-strand breaks (DSBs) has been extensively investigated in interphase cells. Here, we show that mitotic cells treated with DSB-inducing agents activate a "primary" DNA damage response (DDR) comprised of early signaling events
Mammalian tissue- and/or time-specific transcription is primarily regulated in a combinatorial fashion through interactions between a specific set of transcriptional regulatory factors (TRFs) and their cognate cis-regulatory elements located in the regulatory regions. In exploring the DNA regions and TRFs
Human vaccines & immunotherapeutics, 11(4), 998-1009 (2015-03-25)
Human diploid cell strains (HDCSs), possessing identical chromosome sets known to be free of all known adventitious agents, are of great use in developing human vaccines. However it is extremely difficult to obtain qualified HDCSs that can satisfy the requirements
International journal of hematology, 87(3), 239-245 (2008-03-05)
Although coculture of hematopoietic stem cells (HSCs) with stromal cells is a useful system to study hematopoiesis in the niche, little is known regarding the precise cellular and molecular mechanisms of maintaining HSCs through cell-cell interactions. The murine preadipose stromal
The axon initial segment (AIS) is critical for the initiation and propagation of action potentials. Assembly of the AIS requires interactions between scaffolding molecules and voltage-gated sodium channels, but the molecular mechanisms that stabilize the AIS are poorly understood. The
A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.
Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.
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