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M0275

Sigma-Aldrich

Terreno essenziale minimo di Eagle

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10x

Sinonimo/i:

MEM

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100 ML
41,40 €
500 ML
83,30 €

41,40 €


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Cambia visualizzazione
100 ML
41,40 €
500 ML
83,30 €

About This Item

Codice UNSPSC:
12352207
NACRES:
NA.71

41,40 €


Per informazioni sulla disponibilità, contatta il Servizio Clienti.

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Nome del prodotto

Terreno essenziale minimo di Eagle, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Sterilità

sterile-filtered

Stato

liquid

Concentrazione

10 ×

tecniche

cell culture | mammalian: suitable

Impurezze

endotoxin, tested

Componenti

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Applicazioni

Generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. The L-glutamine and sodium bicarbonate are added to the medium after dilution to 1x and pH adjustment.
Minimum Essential Medium Eagle has been used for the formulation of collagen methacrylamide (hydrogel).[1][2][3][4] It has also been used to maintain articular cartilage of bovine knee joints, which was later used for MRI (magnetic resonance imaging) study and histological analysis.[5]

Ricostituzione

Supplement with 0.292 g/L L-glutamine, 2.2 g/L sodium bicarbonate at 1×.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine
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Diffusion tensor imaging of articular cartilage as a measure of tissue microstructure
Meder R, et al.
Osteoarthritis and Cartilage, 14(9), 875-881 (2006)
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Gurkan UA, et al.
Journal of Biomedical Materials Research Part A, 94(4), 1070-1079 (2010)

Articoli

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

Domande

  1. If I have 10x Medium, how can I dilute it in order to have 1x and grow cells?

    1 risposta
    1. Since certain components in 10X medium concentrates precipitate at pH 7.0, it is necessary to adjust the pH of these solutions to maintain solubility.
      Therefore, it may be necessary for the user to adjust the pH of the 1X preparation with sterile 1 N NaOH or 1 N HCl. Procedure For Dilution to 1X (1 Liter):
      (NOTE: Dilution of 10X solutions should be performed with sterile containers, components, and equipment.)
      • Aseptically measure out approximately 850 ml of tissue culture grade water into an appropriate size container.
      • While gently stirring the water, add 100 ml of 10X medium.
      • To the solution, add the required amount of sodium bicarbonate ( 0.35 g/L) and L-glutamine (0.1 g/L), at 1X
      • While stirring, adjust the solution to desired pH with 1 N NaOH or 1 N HCl.
      • Bring medium to final volume with additional tissue culture grade water.
      • It is recommended to sterile filter the prepared medium through a 0.22 ?m sterile membrane, or, for example, a Stericup.
      • Aseptically dispense into sterile containers. Store refrigerated at 2-8° C

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Recensioni

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