Passa al contenuto
Merck
Tutte le immagini(1)

Documenti

L9379

Sigma-Aldrich

Lectin from Phytolacca americana (pokeweed)

lyophilized powder

Sinonimo/i:

PWM, Pokeweed mitogen

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Numero MDL:
MFCD00161973
Codice UNSPSC:
12352202
NACRES:
NA.32

Sterilità

aseptically filled

Livello qualitativo

200

Forma fisica

lyophilized powder

Potenza

≤0.3 μg/mL mitogenic activity

Temperatura di conservazione

2-8°C

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

Lectins are carbohydrate-binding proteins, omnipresent, found in fungi, plants and animals. The structure of lectin is diversely studied in plants and animals. The secondary structure of this protein is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Applicazioni

Lectin from Phytolacca americana (pokeweed) has been used:
  • as a medium supplement for mononuclear cell incubation, to induce lymphocyte proliferation
  • as a positive control in the study of the role of chemokine (C–C motif) ligand 2 (CCL2) in proliferation and apoptosis of decidual leukocytes
  • as a supplement in the RPMI (Roswell park memorial institute) 1640 medium for peripheral blood lymphocyte cultures

Azioni biochim/fisiol

Lectin is known to be useful in glycoconjugate characterizing, imaging and targeting. Its use in microarray assay, enable efficient glycome profiling. This is because of its specific interaction with oligosaccharides, glycoproteins and glycolipids. In plants and fungi, lectin defends against pathogens/feeders. Lectin participates in host recognition and tissue adhesion, thereby aids in the pathogenesis of microorganism.
PWM is extracted from pokeweed roots. This protein has hemagglutinating, leukoagglutinating, and mitogenic properties. PWM is not blood group specific, but has affinity for N-acetyl-β-D-glucosamine oligomers.

Stato fisico

Partially purified TCA precipitate.
Essentially salt-free.

Risultati analitici

Mitogenic activity was determined by BrdU incorporation in HeLa cell cultures.

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H317,H334

Classi di pericolo

Resp. Sens. 1 - Skin Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

The use of a novel combination of diagnostic molecular and cytogenetic approaches in horses with sexual karyotype abnormalities: a rare case with an abnormal cellular chimerism
Demyda P, et al.
Theriogenology, 81(8), 1116-1122 (2014)
Assessment of cellular immune responses of healthy and diseased Tasmanian devils (Sarcophilus harrisii)
Kreiss A, et al.
Developmental and Comparative Immunology, 32(5), 544-553 (2008)
B Hejdeman et al.
HIV medicine, 4(2), 101-110 (2003-04-19)
To study the impact of effective highly active antiretroviral therapy (HAART) on the preservation of long-term CD4 memory cells induced by vaccines in HIV-1-infected patients. Thirty HIV-1-positive patients on HAART with undetectable viral load were randomized into three groups: 10
W E Müller et al.
European journal of biochemistry, 183(2), 391-396 (1989-08-01)
Previously it was established [Pahwa, S., Pahwa, R., Saxinger, C., Gallo, R. C. & Good, R. A. (1985) Proc. Natl. Acad. Sci. USA 82, 8198] that nonviable preparations of human immunodeficiency virus 1 (HIV-1) abolish the proliferative response of human
Nishaki Mehta et al.
Vaccine, 28(21), 3672-3678 (2010-04-02)
Hepatitis B-specific memory B cell (HSMBC) frequencies were measured following hepatitis B vaccination in 15 HIV uninfected and 12 HIV infected adolescents. HSMBC were detected at significantly lower frequencies in HIV infected than in HIV uninfected individuals. The detection of
Visualizza tutti i documenti correlati

Articoli

Lectin Selection Guide: A Useful Guide for Selecting the Right Lectin

Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.