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T0440

Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

peroxidase substrate, chromogenic, liquid

Sinonimo/i:

TMB

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About This Item

Codice UNSPSC:
12352204
NACRES:
NA.83

product name

Sistema a substrato liquido 3,3′,5,5′-Tetrametil-benzidina (TMB) per ELISA, peroxidase substrate

Livello qualitativo

Forma fisica

liquid

Temperatura di conservazione

2-8°C

Descrizione generale

TMB substrate is a chromogenic substrate for horseradish peroxidase - HRP.

Applicazioni

TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The reaction is stopped with acid, forming a yellow reaction product which enables accurate intensity measurement at 450 nm.

How to use our TMB substrate:
  • Our TMB substrate is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase.
  • Upon reaching your assay endpoint with addition of acid, measure the TMB substrate intensity at 450 nm.
  • Our TMB Substrate is recommended for ELISA (microwell) procedures, and has been used in various assays, as a substrate for horseradish peroxidase antibody conjugate for detecting hendra virus glycoprotein, tetracosactide, sIgA and neutrophil extracellular traps (NETs).
  • ′′ Since TMB substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting.
Questo substrato è molto sensibile alla luce e deve essere protetto dalla luce solare diretta o da fonti UV.

Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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