This product is a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. The concentration of the TMB and the formulation of the buffer are considered proprietary.
T0440
3,3′,5,5′-Tetramethylbenzidine
peroxidase substrate, chromogenic, liquid
Sinonimo/i:
TMB
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Cambia visualizzazione
| A voi/SKU | Disponibilità | Prezzo |
|---|---|---|
100 mL | Disponibile per la spedizione OGGIdaSchnelldorf Distribution | 218,00 € |
1 L | Disponibile per la spedizione OGGIdaSchnelldorf Distribution | 1.610,00 € |
5 x 1 L | Disponibile per la spedizione OGGIdaSchnelldorf Distribution | 3.630,00 € |
Informazioni su questo articolo
UNSPSC Code:
12352204
NACRES:
NA.83
Servizio Tecnico
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Permettici di aiutartiNome del prodotto
Sistema a substrato liquido 3,3′,5,5′-Tetrametil-benzidina (TMB) per ELISA, peroxidase substrate
Quality Segment
form
liquid
storage temp.
2-8°C
General description
TMB substrate is a chromogenic substrate for horseradish peroxidase - HRP.
Application
TMB substrate is used in ELISA procedures, which utilize horseradish peroxidase conjugates. TMB substrate develops a soluble blue reaction product that can be read at 370 or 655 nm at 370 or 655 nm. The reaction is stopped with acid, forming a yellow reaction product which enables accurate intensity measurement at 450 nm.
How to use our TMB substrate:
How to use our TMB substrate:
- Our TMB substrate is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase.
- Upon reaching your assay endpoint with addition of acid, measure the TMB substrate intensity at 450 nm.
- Our TMB Substrate is recommended for ELISA (microwell) procedures, and has been used in various assays, as a substrate for horseradish peroxidase antibody conjugate for detecting hendra virus glycoprotein, tetracosactide, sIgA and neutrophil extracellular traps (NETs).
- ′′ Since TMB substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting.
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| Quality Level 300 | Quality Level 100 | Quality Level - | Quality Level 200 |
| form liquid | form liquid | form liquid | form aqueous solution |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
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flash_point_f
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Classe di stoccaggio
12 - Non Combustible Liquids
Elenchi normativi
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54827-17-7
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I want to know about T0440 product concentration
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How long should I develop? And what is the ideal OD between .1 and 1.0?
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There is no set time at which the reaction must be stopped. Depending on the peroxidase activity measured, the color may form very quickly or slowly. Kindly adhere to the instructions provided in our datasheet as closely as possible.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/277/866/t0440dat-ms.pdfUtile?
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What is the limit of detection for Product No. T0440, the 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System used in ELISA?
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The limit of detection for Product No. T0440, the 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System used in ELISA, is specified to range from 3 to 6 pg/ml.
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What is the difference between products T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA, and T8665, 3,3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System?
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Both products are comparable and either one can be used in ELISA procedures. The formulations which are proprietary may have some slight differences.
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What is the concentration of the TMB and what is the solvent in Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
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The concentration of TMB in this formulation and the identity of the solvent are considered proprietary information. The product is a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer.
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Do I need to dilute Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
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No, the product should not be diluted.. The product is ready-to-use for ELISA applications. If the intensity of the reaction needs to be reduced, it is recommended that the antibodies or conjugates be diluted. Usage information is described on our data sheet.
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Do I need to add hydrogen peroxide to Product T0440, 3, 3´,5,5´-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA?
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No hydrogen peroxide should be added to product T0440. The product, which contains a peroxidase substrate, already contains hydrogen peroxide. This solution is ready to use for ELISA assays.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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