The antibiotics in the LB-agar plates are not stable. The antibiotic degrades over time, making it ineffective at selecting transformed bacteria grown on the plate. Because of this, we recommend the plates are used as soon as possible once they are received. For labs with low use, the plates may expire quickly, so they may benefit from pouring their own plates using the LB - Miller powder with agar formulation (L3147).
L0418
LB Agar Carbenicillin - 100, Plates
LB Agar Carbenicillin - 100, Plates
pre-poured agar plates with 100 μg/ml carbenicillin
Sinonimo/i:
carbenicillin agar plates
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About This Item
Codice UNSPSC:
41121800
Sterilità:
sterile; autoclaved
Compatibilità:
selective for Escherichia coli
selective for coliforms
selective for coliforms
Prezzi e disponibilità al momento non sono disponibili
Prodotti consigliati
Grado
for molecular biology
Livello qualitativo
Sterilità
sterile; autoclaved
Stato
plates (10 cm)
plates (10cm, 20-25ml each)
Disponibilità
available only in USA
Capacità
20-25 mL
Temperatura di conservazione
2-8°C
Compatibilità
selective for Escherichia coli
selective for coliforms
Categorie correlate
Descrizione generale
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.
Applicazioni
LB Agar Carbenicillin - 100, Plates may be used to culture transformed bacteria with an antibiotic-resistant plasmid using a microwave oven.[1]
Suitable for selective cultivation of E. coli strains that contain plasmids conferring carbenicillin resistance. The bacteria can be used for DNA plasmid production or production of recombinant proteins.
Caratteristiche e vantaggi
Miller′s LB Agar plates provide:
- Convenient, ready-to-use 10cm format
- Standard formulation plus antibiotic
Componenti
15 g/L Agar
10 g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
100 μg/ml carbenicillin
10 g/L Tryptone
10 g/L NaCl
5 g/L Yeast Extract
100 μg/ml carbenicillin
Altre note
The plates must be used within two months of the manufacture date to ensure the antibiotics are fully active. The product may be shipped up until its expiration date but is generally shipped 2-4 weeks before expiration.
Prodotti correlati
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
13 - Non Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Sterile preparation of antibiotic-selective LB agar plates using a microwave oven
Iacoviello Michael P and Rubin SA
Biotechniques, 30(5), 963-965 (2001)
Articoli
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
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Why do the LB-agar plates with antibiotics have such a short expiration date?
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What is the Department of Transportation shipping information for this product?
1 risposta-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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