G4155
β-Galactosidase from Escherichia coli
aqueous glycerol suspension, ≥500 units/mg protein (biuret)
Sinonimo/i:
β-D-Galactoside galactohydrolase, Lactase
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54
Prodotti consigliati
Forma fisica
aqueous glycerol suspension
Livello qualitativo
Attività specifica
≥500 units/mg protein (biuret)
PM
465 kDa
Temperatura di conservazione
−20°C
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Applicazioni
β-Galactosidase is used in the enzymatic assays in the synthesis of imidazolo-pyrrolidinoses.
Azioni biochim/fisiol
β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
Proprietà fisiche
Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)
Definizione di unità
One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.
Stato fisico
Suspension in 50% glycerol, 5 mM Tris buffer salts, 5 mM magnesium chloride, 0.5 mM DTT, 0.5 mM mercaptoethanol. pH 7.4
Altre note
Loss of enzymatic activity can occur if this enzyme is frozen. This enzyme is provided in a 50% glycerol solution and will not freeze if stored at -20° Celsius. It is not recommended that this enzyme preparation be stored in an ultracold freezer (-60 to -100° Celsius).
Inibitore
Substrato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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