N1127

2-Nitrophenyl β-D-galactopyranoside

≥98% (enzymatic)

• Sinonimo/i: ONPG , o-Nitrophenyl β-D-galactopyranoside, ONPG
• Formula empirica (notazione di Hill): C₁₂H₁₅NO₈
• Numero CAS: 369-07-3
• Peso molecolare: 301.25
• Beilstein: 92207
• Numero CE: 206-716-1
• Numero MDL: MFCD00063255
• Codice UNSPSC: 12352200
• ID PubChem: 24897461
• NACRES: NA.52

Proprietà

• Grado: Molecular Biology; for molecular biology
• Saggio: ≥98% (enzymatic)
• Stato: powder
• Temperatura di conservazione: −20°C
• Stringa SMILE: OC[C@H]1O[C@@H](Oc2ccccc2[N+]([O-])=O)[C@H](O)[C@@H](O)[C@H]1O
• InChI: 1S/C12H15NO8/c14-5-8-9(15)10(16)11(17)12(21-8)20-7-4-2-1-3-6(7)13(18)19/h1-4,8-12,14-17H,5H2/t8-,9+,10+,11-,12-/m1/s1; KUWPCJHYPSUOFW-YBXAARCKSA-N

Descrizione

Descrizione generale

ONPG (2-Nitrophenyl β-D-galactopyranoside) is a colorimetric substrate for β-galactosidase.

Applicazioni

2-Nitrophenyl β-D-galactopyranoside is an enzyme substrate used to detect lacZ activity and hence the presence of β-galactosidase.

Azioni biochim/fisiol

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Principio

β-galactosidase, breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides. 2-Nitrophenyl β-D-galactopyranoside hydrolysis results in the release of galactose and a yellow chromogenic compound. The test substrate does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period.

Ricostituzione

A stock solution can be prepared in molecular biology grade water at a concentration of 3 mg/ml. Alternatively, a stock solution of approximately 20.5 mg/ml is prepared in 100 mM sodium phosphate buffer (pH 7.3). Gentle warming may be required to completely dissolve the product.

Substrati

Chromogenic substrate for β-galactosidase

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)