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G3153

Sigma-Aldrich

β-Galactosidase from Escherichia coli

lyophilized powder, ≥500 units/mg protein

Sinonimo/i:

β-D-Galactoside galactohydrolase, Lactase

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About This Item

Numero CAS:
9031-11-2
Classificazione EC (Enzyme Commission):
3.2.1.23 (BRENDA, IUBMB)
Numero CE:
232-864-1
Numero MDL:
MFCD00130623
Codice UNSPSC:
12352204
NACRES:
NA.54

Ricombinante

expressed in E. coli

Livello qualitativo

200

Forma fisica

lyophilized powder

Attività specifica

≥500 units/mg protein

PM

465 kDa

Temperatura di conservazione

−20°C

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Descrizione generale

β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene.

Applicazioni

β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cysteamide).
β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration.

Azioni biochim/fisiol

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.
β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression.

Confezionamento

Package size based on protein content

Proprietà fisiche

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

Definizione di unità

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactopyranoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Stato fisico

Stabilized with phosphate buffer and sucrose

Inibitore

N° Catalogo
Descrizione
Determinazione del prezzo

Substrato

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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USP

USP

1356698

Lactase

Peptide-Mediated Membrane Transport of Macromolecular Cargo Driven by Membrane Asymmetry
Li X, et al.
Analytical Chemistry, 89(22), 12369-12374 (2017)
M J O'Sullivan et al.
Annals of clinical biochemistry, 16(5), 221-240 (1979-09-01)
In the last few years, the use of enzyme labels in immunoassays has been investigated. The aim of this review is to evaluate critically the role of such labels in clinical biochemistry. Special attention has been given to the problems
Samuel Berhanu et al.
Journal of basic microbiology, 62(6), 669-688 (2022-03-16)
pUC18 and pUC19 are well-known high copy-number plasmid vectors routinely used for DNA cloning purposes. We show here that, in Escherichia coli transformed by native pUC18, the α-complementation of β-galactosidase (i.e., mediated by the peptide LacZα18) is intrinsically weak and
Structure of beta-galactosidase at 3.2-AA resolution obtained by cryo-electron microscopy
Bartesaghi A, et al.
Proceedings of the National Academy of Sciences of the USA, 111(32), 11709-11709 (2014)
Application of galactose-sensitive E. coli strains as selective hosts for LacZ-plasmids.
Gossen JA, et al.
Nucleic Acids Research, 20(12), 3254-3254 (1992)
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