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A7130

Sigma-Aldrich

β-Amylase from barley

greener alternative

Type II-B, 20-80 units/mg protein (biuret)

Sinonimo/i:

1,4-α-D-Glucan maltohydrolase

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10000 UNITS
107,00 €
50000 UNITS
428,00 €
250000 UNITS
1.490,00 €

107,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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Cambia visualizzazione
10000 UNITS
107,00 €
50000 UNITS
428,00 €
250000 UNITS
1.490,00 €

About This Item

Numero CAS:
9000-91-3
Classificazione EC (Enzyme Commission):
3.2.1.2 (BRENDA, IUBMB)
Numero CE:
232-566-1
Numero MDL:
MFCD00081391
Codice UNSPSC:
12352204
NACRES:
NA.54

107,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

Tipo

Type II-B

Stato

powder

Attività specifica

20-80 units/mg protein (biuret)

Caratteristiche più verdi

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Categoria alternativa più verde

, Enabling

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Applicazioni

β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides [1]. β -Amylase, from barley, has been used to study how pressure and temperature affect catalytic activity [2].

Azioni biochim/fisiol

β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. Pure, crystalline β-amylase preparation consists of four isoenzymes with different isoelectric points. The enzyme polymerizes very rapidly through the sulfhydryl groups in the absence of reducing agents. p-Chloromercuribenzoate inhibits the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can completely restore the activity.[3]

Qualità

Crude

Definizione di unità

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C.

Altre note

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P342 + P311

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Certificati d'analisi (COA)

Lot/Batch Number
0000416595
0000416595
0000401460
0000401459
0000368825

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Volker Heinz et al.
Biotechnology progress, 21(6), 1632-1638 (2005-12-03)
The depolymerization of starch by beta-amylase during exposure to hydrostatic pressure up to 700 MPa and within a temperature range from 20 to 70 degrees C has been investigated. Inactivation of the enzyme as well as alterations in conversion speed
Purification and characterisation of crystalline -amylase from barley.
K Visuri et al.
European journal of biochemistry, 28(4), 555-565 (1972-08-04)
Claudinéia Aparecida Soares et al.
Journal of agricultural and food chemistry, 59(12), 6672-6681 (2011-05-20)
Different banana cultivars were used to investigate the influences of starch granule structure and hydrolases on degradation. The highest degrees of starch degradation were observed in dessert bananas during ripening. Scanning electron microscopy images revealed smooth granule surface in the
Heike Reinhold et al.
The Plant cell, 23(4), 1391-1403 (2011-04-14)
Plants contain β-amylase-like proteins (BAMs; enzymes usually associated with starch breakdown) present in the nucleus rather than targeted to the chloroplast. They possess BRASSINAZOLE RESISTANT1 (BZR1)-type DNA binding domains--also found in transcription factors mediating brassinosteroid (BR) responses. The two Arabidopsis
Déborah LeCorre et al.
Biomacromolecules, 13(1), 132-137 (2011-12-03)
Starch nanocrystals (SNCs) are crystalline platelets resulting from the acid hydrolysis of starch. A limiting factor for their more widespread use is their preparation duration. Therefore, this study investigates the possibility of developing an enzymatic pretreatment of starch to reduce
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Protocolli

Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)

Beta-amylase is found in bacteria, fungi, and plants. To measure β-amylase activity, this assay uses a colorimetric spectrophotometric stop reaction at 540 nm.

Domande

Recensioni

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