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A6255

Sigma-Aldrich

α-Amylase from porcine pancreas

greener alternative

PMSF Treated, Type I-A, saline suspension, ≥1000 units/mg protein (E1%/280)

Sinonimo/i:

β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, porcine pancreas α-amylase

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About This Item

Classificazione EC (Enzyme Commission):
3.2.1.1 (BRENDA, IUBMB)
Numero CE:
232-565-6
Numero MDL:
MFCD00081319
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54

Origine biologica

Porcine pancreas

Livello qualitativo

200

Tipo

Type I-A

Forma fisica

saline suspension

Attività specifica

≥1000 units/mg protein (E1%/280)

PM

51-54 kDa

Caratteristiche più verdi

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

Categoria alternativa più verde

, Enabling

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

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Categorie correlate

Descrizione generale

Molecular mass: 51-54 kDa.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Applicazioni

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A6255 is from porcine pancreas and is type I-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis .

Azioni biochim/fisiol

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca2+ necessary for stability.

Definizione di unità

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Stato fisico

Suspension in 2.9 M NaCl solution containing 3 mM CaCl2

Nota sulla preparazione

PMSF treated. 2× crystallized

Altre note

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Inibitore

N° Catalogo
Descrizione
Determinazione del prezzo

D9305

1-Deoxynojirimycin hydrochloride

Substrato

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

Enzymatic Assay of α-Amylase (EC 3.2.1.1)

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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