An essential product for immunoprecipitations of VSV-tagged recombinant proteins. Given the packaging of the product, we aliquoted it into microfuge tubes on arrival and stored it at 4 C.
A1970
Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal
Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal
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clone P5D4, purified from hybridoma cell culture, PBS suspension
Sinonimo/i:
Monoclonal Anti-VSV Glycoprotein
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1 ML
625,00 €
625,00 €
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Cambia visualizzazione
1 ML
625,00 €
About This Item
Codice UNSPSC:
12352203
NACRES:
NA.56
625,00 €
Per informazioni sulla disponibilità, contatta il Servizio Clienti.
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Coniugato
agarose conjugate
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
P5D4, monoclonal
Stato
PBS suspension
Classi chimiche degli analiti
proteins (VSV-G)
tecniche
immunoprecipitation (IP): suitable
protein purification: suitable
Isotipo
IgG1
Capacità
≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein)
Condizioni di spedizione
wet ice
Temperatura di conservazione
2-8°C
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Descrizione generale
Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose.
Specificità
The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.
Immunogeno
synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.
Applicazioni
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.
Stato fisico
Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
Nota sulla preparazione
Prepared using cyanogen bromide-activated agarose.
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Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
nwg
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Larry A Gallagher et al.
Nature microbiology, 7(6), 844-855 (2022-06-02)
DNA-protein interactions are central to fundamental cellular processes, yet widely implemented technologies for measuring these interactions on a genome scale in bacteria are laborious and capture only a snapshot of binding events. We devised a facile method for mapping DNA-protein
Sachin Mohan et al.
The Journal of biological chemistry, 285(45), 34566-34578 (2010-08-26)
The small intestinal BB Na(+)/H(+) antiporter NHE3 accounts for the majority of intestinal sodium and water absorption. It is highly regulated with both postprandial inhibition and stimulation sequentially occurring. Phosphatidylinositide 4,5-bisphosphate (PI(4,5)P(2)) and phosphatidylinositide 3,4,5-trisphosphate (PI(3,4,5)P(3)) binding is involved with
Yael Katan-Khaykovich et al.
Proceedings of the National Academy of Sciences of the United States of America, 108(4), 1296-1301 (2011-01-12)
Nucleosome deposition occurs on newly synthesized DNA during DNA replication and on transcriptionally active genes via nucleosome-remodeling complexes recruited by activator proteins and elongating RNA polymerase II. It has been long believed that histone deposition involves stable H3-H4 tetramers, such
Andrew M Lippa et al.
Molecular microbiology, 115(6), 1138-1151 (2020-11-28)
The H-NS-like proteins MvaT and MvaU act coordinately as global repressors in Pseudomonas aeruginosa by binding to AT-rich regions of the chromosome. Although cells can tolerate loss of either protein, identifying their combined regulatory effects has been challenging because the
Bridget R Kulasekara et al.
eLife, 2, e01402-e01402 (2013-12-19)
Individual cell heterogeneity is commonly observed within populations, although its molecular basis is largely unknown. Previously, using FRET-based microscopy, we observed heterogeneity in cellular c-di-GMP levels. In this study, we show that c-di-GMP heterogeneity in Pseudomonas aeruginosa is promoted by
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