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EAGLETMMRO

Roche

EagleTaq Universal Master Mix (ROX)

sufficient for 100 reactions, sufficient for 500 reactions, sufficient for 5000 reactions, suitable for qPCR, suitable for RT-qPCR

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500 X 20 REACTIONS
417,00 €

417,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.
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500 X 20 REACTIONS
417,00 €

About This Item

Codice UNSPSC:
41106300
NACRES:
NA.55

417,00 €

Per informazioni sulla disponibilità, contatta il Servizio Clienti.

impiego

sufficient for 100 reactions
 sufficient for 500 reactions
 sufficient for 5000 reactions

Livello qualitativo

100

Caratteristiche

dNTPs included
hotstart

Produttore/marchio commerciale

Roche

Confezionamento

pkg of 100 x 20 μL reactions (07260288190)
pkg of 500 x 20 μL reactions (07260296190)
pkg of 5000 x 20 μL reactions (07249926190)

tecniche

RT-qPCR: suitable
qPCR: suitable

input

purified DNA

Metodo di rivelazione

probe-based

Descrizione generale

2× concentrated, ready-to-use hot start master mix for qPCR and qRT-PCR using the hydrolysis probe detection format on real-time PCR instruments.

The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated PCR master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR hydrolysis probe reactions. It contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
The EagleTaq Universal Master Mix (ROX) contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
The hot start properties allow reaction setup at ambient temperature.
With this robust reagent, any PCR protocol optimization is minimized
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated polymerase chain reaction (PCR) master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR (qPCR) and quantitative reverse transcription PCR (qRT-PCR) hydrolysis probe reactions.

Applicazioni

EagleTaq Universal Master Mix (ROX) has been used in quantitative, real-time polymerase chain reaction (qPCR )[1][2] and quantitative reverse transcription PCR (qRT-PCR)[1][3].

Caratteristiche e vantaggi

  • The EagleTaq Universal Master Mix (ROX) contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
  • It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
  • The hot start properties allow reaction setup at ambient temperature.
  • With this robust reagent, any PCR protocol optimization is minimized.

Componenti

Contains EagleTaq DNA Polymerase and a reaction buffer with dATP, dCTP, dGTP, dUTP, MgCl2, and ROX.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

does not flash

Punto d’infiammabilità (°C)

does not flash

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Oncology reports, 45(4) (2021-03-03)
The precise mechanism of intercellular communication between cancer cells following radiation exposure is unclear. Exosomes are membrane‑enclosed small vesicles comprising lipid bilayers and are mediators of intercellular communication that transport a variety of intracellular components, including microRNAs (miRNAs or miRs).
Hiroto Suwa et al.
Biochemistry and biophysics reports, 8, 62-67 (2016-08-12)
Retinoic acid (RA) is a metabolite of vitamin A and has important roles in development, differentiation, and reproduction. Activin has been shown to regulate the RA pathway and affect granulosa cell (GC) proliferation, suggesting that RA is important for early
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Proceedings of the National Academy of Sciences of the United States of America, 116(21), 10525-10530 (2019-05-10)
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Articoli

PCR Master Mix

PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Contenuto correlato

PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

Applicazioni della PCR

La PCR (reazione a catena della polimerasi) è una tecnica che consente di amplificare le molecole di acido nucleico comunemente usata in molte applicazioni fra cui RT-PCR, hot start PCR, end point PCR ed altre.

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