11699121001

PCR Buffer Set

suitable for PCR, pkg of 2 × 2 mL

• Codice UNSPSC: 41116133

Proprietà

• Confezionamento: pkg of 2 × 2 mL
• Produttore/marchio commerciale: Roche
• tecniche: PCR: suitable
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

The kit-provided PCR buffer without MgCl₂, in combination with the kit-provided MgCl₂ stock solution, are used for the individual adjustment of the Mg²⁺ concentration. In most PCR applications, a concentration of 1.5 mM MgCl₂ will yield satisfactory results with a dNTP concentration of 200 μM for each nucleotide. To optimize the PCR, titrate to determine the optimal Mg²⁺ concentration for high PCR specificity and amplicon yield.

Applicazioni

For optimization of the magnesium-chloride concentration in the polymerase chain reaction (PCR) using Taq DNA Polymerase.

Confezionamento

1 set containing 2 components

1 set containing 3 components

Qualità

Function test: Each lot of buffer is tested in PCR.
Absence of contaminants: No exo- or endonuclease activity is detectable according to the current Quality Control procedures.

Altre note

For life science research only. Not for use in diagnostic procedures.

Solo come componenti del kit

• MgCl₂ Stock Solution 25 mM
• PCR Buffer without Mg²⁺ 10x concentrated
• Alkaline Phosphatase Substrate, CSPD
• Enhancer, Emerald II
• Assay Buffer

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash