11681451001
Roche
NBT/BCIP Stock Solution
Sinonimo/i:
5-bromo-4-chloro-3-indoyl phosphate, BCIP
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About This Item
Codice UNSPSC:
12352204
Prodotti consigliati
Forma fisica
solution
Livello qualitativo
PM
817.7
Confezionamento
pkg of 8 mL
Produttore/marchio commerciale
Roche
Temperatura di conservazione
2-8°C
Descrizione generale
The reaction product has a blue color and is insoluble in water. The blue color can be visually seen.
Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N
Evaluation: visually
Formulas: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N
Evaluation: visually
Applicazioni
- The solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, including: Southern blot
- Northern blot
- Western blot
- Colony and plaque lifts
- In situ hybridization
- and for applications in immunohistochemistry and immunocytochemistry.
Stato fisico
Solution of 18.75 mg/ml nitro blue tetrazolium chloride and 9.4 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in 67% (DMSO) (v/v).
Note: The color of the solution can vary from yellowish to light brown.
Note: The color of the solution can vary from yellowish to light brown.
Nota sulla preparazione
Working solution: Preparation of staining solution
Always prepare this solution fresh shortly before use.
Always prepare this solution fresh shortly before use.
- For all applications except DIG System: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl, 0.05 M MgCl2.
- For DIG System applications: Add 200 μl of the stock solution to 10 ml 0.1 M Tris-HCl, pH 9.5 (20 °C), 0.1 M NaCl
Altre note
For life science research only. Not for use in diagnostic procedures.
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
does not flash
Punto d’infiammabilità (°C)
does not flash
Certificati d'analisi (COA)
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Protocolli
NBT/BCIP Stock Solution Protocol & Troubleshooting
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