565322-U

Ascentis^® RP-Amide (3 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Szinonimák: RP-Amide Reversed-Phase Chromatography Column
• UNSPSC kód: 41115700
• eCl@ss: 32110501
• NACRES: SB.52
• H × belső átmérő: 15 cm × 4.6 mm
• külső felület: 450 m²/g
• részecskeméret: 3 μm
• mátrix aktív csoport: amide, alkyl phase
• pórusméret: 100 Å
• Mátrix: fully porous particle; silica gel high purity, spherical

Tulajdonságok

• Terméknév: Ascentis^® RP-Amide HPLC Column, 3 μm particle size, L × I.D. 15 cm × 4.6 mm
• material: stainless steel column
• Minőségi szint: 100
• Ügynökség: suitable for USP L60
• termékcsalád: Ascentis^®
• Jellemzők: endcapped
• gyártó/kereskedő neve: Ascentis^®
• kiszerelés: 1 ea of
• a címkézés mértéke: 19.5% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technika/technikák: HPLC: suitable; LC/MS: suitable
• H × belső átmérő: 15 cm × 4.6 mm
• külső felület: 450 m²/g
• felület bevonatolás: 2.7 μmol/m²
• szennyeződések: <5 ppm metals
• Mátrix: fully porous particle; silica gel high purity, spherical
• mátrix aktív csoport: amide, alkyl phase
• részecskeméret: 3 μm
• pórusméret: 100 Å
• Üzemi pH tartomány: 2-8
• alkalmazás(ok): food and beverages
• elválasztástechnika: reversed phase

Leírás

Általános leírás

The Ascentis family of columns is the fourth generation of HPLC column technology from Supelco scientists. Ascentis columns are bonded on high purity, 100 Angstrom silica including 3, 5, and 10 micron particle size. Columns are designed for small molecule applications and are scalable from micro columns (1.0 mm I.D.) to preparative dimensions (50 mm I.D.). The family includes C18, C8, Phenyl, Si and embedded polar group phase, RP-Amide.

Ascentis RP-Amide is a new generation ultra low bleed, embedded polar group (EPG) phase that provides orthogonal selectivity and increased resolution for HPLC and LC-MS analysis of polar compounds. The Ascentis RP-Amide is the first choice in embedded polar group HPLC phases.

Alkalmazás

• Preparation of mixed-mode stationary phase for separation of peptides and proteins in high-performance liquid chromatography: Highlights the application of Ascentis^® RP-Amide HPLC column in the development of mixed-mode stationary phases, further demonstrating its versatility in handling diverse biochemical compounds and its pivotal role in advancing pharmaceutical and proteomic research (Alharthi et al., 2022).


• On-line hyphenation of solid-phase extraction to chromatographic separation of sulfonamides with fused-core columns in sequential injection chromatography: Showcases the efficacy of Ascentis^® RP-Amide HPLC columns in environmental analysis, specifically for the detection of contaminants in complex matrices. This study underscores the column′s robustness and high-resolution capabilities, making it invaluable in environmental laboratories (Batista et al., 2015).


• Determination of zearalenone and its metabolites in endometrial cancer by coupled separation techniques: Demonstrates the critical role of Ascentis^® RP-Amide HPLC columns in clinical research, particularly in the sensitive and accurate measurement of biomarkers in cancer diagnostics. The column′s superior retention for polar compounds proves essential for precise biomedical analyses (Gadzała-Kopciuch et al., 2011).

Tulajdonságok és előnyök

• Excellent retention and peak shape for polar compounds
• 100% aqueous compatibility
• Ultra low bleed, LC-MS compatible
• Unique selectivity

Javasolt termékek

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Jogi információk

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Biztonsági adatok

• Tárolási osztály kódja: 13 - Non Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable