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Merck

ZRB1745

Sigma-Aldrich

Anti-TMEM206 Antibody, clone 2H13 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Szinonimák:

Proton-activated chloride channel;PAC;Transmembrane protein 206

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352203
NACRES:
NA.43

biológiai forrás

rabbit

Minőségi szint

rekombináns

expressed in HEK 293 cells

konjugátum

unconjugated

antitest forma

purified antibody

antitest terméktípus

primary antibodies

klón

2H13, recombinant monoclonal

leírás

recombinant, expressed in HEK 293 cells

termékcsalád

ZooMAb® learn more

form

lyophilized

molekulatömeg

calculated mol wt 40.04 kDa
observed mol wt ~48 kDa

tisztítva

using Protein A

faj reaktivitás

mouse, human, rat

kiszerelés

antibody small pack of 25 μL

környezetbarátabb alternatív termék tulajdonságai

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

fejlettebb validálás

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technika/technikák

ELISA: suitable
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable

izotípus

IgG

epitóp szekvencia

N-terminal

Protein ID elérési szám

UniProt elérési szám

környezetbarátabb alternatív kategória

kiszállítva

ambient

tárolási hőmérséklet

2-8°C

célzott transzláció utáni módosítás

unmodified

Géninformáció

human ... PACC1(55248)

Általános leírás

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Egyediség

Clone 2H13 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Transmembrane protein 206 (TMEM206). It targets an epitope within 24 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 24 amino acids from the N-terminal region of human Transmembrane protein 206 (TMEM206).

Alkalmazás

Quality Control Testing

Evaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TMEM206 in Human brain tissue lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected TMEM206 in lysates from Mouse brain tissue and PC12 cells, and recombinant Human TMEM206 protein.

Enzyme Immunoassay Analysis (ELISA): Serial dilutions from a representative lot detected TMEM206 protein.

Affinity Binding Assay: A representative lot of this antibody bound TMEM206 peptide with a KD of 1.0 x 10-12 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TMEM206 in HeLa, A431, and NIH 3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in Human brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected TMEM206 in Human brain tissue lysate.

Cél megnevezése

Proton-activated chloride channel (UniProt: Q9H813; also known as PAC, Transmembrane protein 206) is encoded by the PACC1 (also known as TMEM206) gene (Gene ID: 55248) in human. Chloride ions (Cl ) are the most abundant anions in animal cells and their movement across cell membrane is mediated by Cl channels and transporters, which are essential for several cellular functions, such as regulation of cell volume, acidification of intracellular vesicles, and excitability control in muscle cell. TMEM206 is an evolutionarily conserved, multi-pass membrane glycoprotein with two cytoplasmic domains, two transmembrane domains, and one extracellular domain. It is widely expressed, but its highest expression is observed in the brain. It is an essential component of the acid-sensitive outwardly rectifying (ASOR) anion channel that is involved in import of chloride ions in response to extracellular acidic pH. It is also involved in acidosis-induced cell death by mediating chloride influx and subsequent cell swelling. TMEM206 -/- cells exhibit partial protection from acid-induced cell death. Higher expression of TMEM206 has been observed in colorectal cancer (CRC), breast, and liver cancers. Overexpression of TMEM206 promotes proliferation and invasion in CRC cells and up-regulates the levels of phospho-AKT and its downstream signaling components. This ZooMAbZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Deng, Z., et al. (2021). Science Adv. 7(9); eabe5983; Zhang, L., et al. (2020). Oncol. Lett. 5; 174; Ullrich, F., et al. (2019). eLife. 8; e49187).

Fizikai forma

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Feloldás

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Tárolás és stabilitás

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Jogi információk

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 1


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