SRP5190

HSP70, His tagged human

recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Szinonimák: HSP70-1, HSP72, HSPA1, HSPA1A, HSPA1B
• CAS-szám: 2071612-66-1
• UNSPSC kód: 12352200
• NACRES: NA.32

Tulajdonságok

• biológiai forrás: human
• rekombináns: expressed in baculovirus infected Sf9 cells
• Teszt: ≥70% (SDS-PAGE)
• Forma: buffered aqueous glycerol solution
• molekulatömeg: ~70 kDa
• NCBI elérési szám: NM_005345
• alkalmazás(ok): cell analysis
• kiszállítva: dry ice
• tárolási hőmérséklet: −70°C
• Géninformáció: human ... HSPA1A(3303)

Leírás

Általános leírás

Heat shock protein 70 (HSP70) is a ubiquitous molecular chaperone. It comprises an N-terminal domain, nucleotide-binding domain (NBD), substrate-binding domain (SBD), and a C-terminal domain.

Alkalmazás

Heat shock protein 70 (HSP70), His tagged human has been used:

• to study the interaction between HSP70 and receptor of advanced glycation endproducts (RAGE) using protein proximity ligand assay (PLA)
• to facilitate the import of superoxide dismutase 2 (SOD2) into the mitochondria
• to study its role in muscle catabolism

Biokémiai/fiziológiai hatások

Heat shock protein 70 (HSP70) plays a role in the cellular protein folding and remodeling process. It is also involved in the translocation of polypeptides into the chloroplast, mitochondria, and endoplasmic reticulum. HSP70 facilitates dismantling of protein complexes and modulates protein activity. It also plays a role in guarding cells from proteotoxic stress, pathophysiological conditions, and organismal aging. HSP70 participates in the activation of several immune cells such as macrophages, natural killer (NK) cells, B lymphocytes, peripheral monocytes, and antigen-presenting cells (APCs).

Fizikai forma

Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.

Elkészítési megjegyzés

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Biztonsági adatok

• Piktogramok: GHS08,GHS07
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H315,H319,H360D
• Óvintézkedésre vonatkozó mondatok: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Veszélyességi osztályok: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Tárolási osztály kódja: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable