SRP5117
PCNA, His tagged human
recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution
Szinonimák:
MGC8367
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
Javasolt termékek
biológiai forrás
human
rekombináns
expressed in E. coli
Teszt
≥70% (SDS-PAGE)
form
buffered aqueous glycerol solution
molekulatömeg
~37 kDa
NCBI elérési szám
alkalmazás(ok)
cell analysis
kiszállítva
dry ice
tárolási hőmérséklet
−70°C
Géninformáció
human ... PCNA(5111)
Általános leírás
PCNA (proliferating cell nuclear antigen) gene is mapped to human chromosome 20p12.3 and encodes a scaffolding protein. PCNA is a homotrimeric protein known to localize in the nucleus. The PCNA ring has a central cavity that is large enough to allow the dsDNA to slide freely along it. PCNA is a nuclear protein whose appearance correlates with the proliferative state of the cells and is a cofactor of DNA polymerase delta.
Biokémiai/fiziológiai hatások
Proliferating cell nuclear antigen (PCNA) significantly maintains the longevity of mature neutrophil granulocytes. Upregulation of the PCNA gene is observed in cervical squamous cell carcinoma. PCNA supports cell survival by elevating metabolism flux. PCNA expression is known to offer resistance to chemotherapy. PCNA is a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Immunofluorescence studies have shown that p300 may play a role in DNA repair synthesis through its interaction with PCNA. In vitro and in vivo p300 forms a complex with PCNA that does not depend on the S phase of the cell cycle and stimulates DNA synthesis in vitro. PCNA interacts with the Williams syndrome transcription factor (WSTF) allowing it to target to DNA replication foci, that then allows recruitment of SNF2H.
Fizikai forma
Supplied in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
Elkészítési megjegyzés
after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
Tárolási osztály kódja
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
WGK
WGK 1
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
Már rendelkezik ezzel a termékkel?
Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.
Cytoplasmic proliferating cell nuclear antigen connects glycolysis and cell survival in acute myeloid leukemia
Ohayon D, et al.
Scientific reports, 6(3), 35561-35561 (2016)
Proliferating cell nuclear antigen prevents trinucleotide repeat expansions by promoting repeat deletion and hairpin removal
Beaver JM, et al.
DNA Repair, 48(2), 17-29 (2016)
S Hasan et al.
Nature, 410(6826), 387-391 (2001-03-27)
The transcriptional coactivator p300 interacts with many transcription factors that participate in a broad spectrum of biological activities, such as cellular differentiation, homeostasis and growth control. Mouse embryos lacking both p300 alleles die around mid-gestation, with pleiotropic defects in morphogenesis
Promoter methylation of GATA4, WIF1, NTRK1 and other selected tumour suppressor genes in ovarian cancer
Chmelarova M, et al.
Folia Biologica (Praha.), 59(2), 87-87 (2013)
Hisham Mazal et al.
eLife, 11 (2022-05-27)
Cryogenic optical localization in three dimensions (COLD) was recently shown to resolve up to four binding sites on a single protein. However, because COLD relies on intensity fluctuations that result from the blinking behavior of fluorophores, it is limited to
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