SRE0117

Salt Active Nuclease (SAN)

High Purity, recombinant, expressed in Pichia pastoris, Triton-free

• Szinonimák: endonuclease, salt active endonuclease, salt tolerant nuclease
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• rekombináns: expressed in Pichia pastoris
• Minőségi szint: 200
• összetétel: Triton-free
• kiszerelés: pkg of 25 kU
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

This Salt Active Nuclease (SAN) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.

Alkalmazás

• Useful for purification of tagged recombinant proteins
• Manufacturing of nucleic acid-free molecular enzymes and reagents
• Research of vaccines, viruses and antibodies

Tulajdonságok és előnyök

• Active at low temperatures
• Binds strongly to cation exchange media because of high isoelectric point, even at elevated pH and salt concentrations where few other proteins show binding
• Typically does not bind to anion exchange or IMAC media
• Detergent free No protease detected

Egység definíció

One unit is defined as the amount of enzyme that causes a ΔA260 = 1.0 in 30 minutes at 37 °C in 25 mM Tris-HCI pH 8.5 (@25 °C), 5 mM MgCl₂, 500 mM NaCl, and 50 µg/mL calf thymus DNA.

Feloldás

In solution

Egyéb megjegyzések

• Temperature: 5 – 40°C, optimal: ~35°C
• Salt concentration (NaCl / KCl): 150 - 900 mM, optimal: 400 - 650 mM
• Mg²⁺: >1 mM is required for activity, optimal 5 - 50 mM
• pH: 7.3 – 10.0, optimal 8.2 - 9.2

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 2
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable