SAB4200780

Anti-Human IgM (μ-chain specific) antibody, Mouse monoclonal

clone MB-11, purified from hybridoma cell culture

• Szinonimák: Anti-Human immunoglobulin M
• UNSPSC kód: 12352203
• NACRES: NA.46
• klón: MB-11, monoclonal
• application: ELISA
• faj reaktivitás: human
• technika/technikák: ELISA: 0.1-0.2 μg/mL using 2.5 μg/mL Human IgM myeloma for coating; immunoblotting: suitable
• citations: 1

Tulajdonságok

• biológiai forrás: mouse
• Minőségi szint: 300
• antitest forma: purified from hybridoma cell culture
• antitest terméktípus: primary antibodies
• klón: MB-11, monoclonal
• Forma: buffered aqueous solution
• faj reaktivitás: human
• koncentráció: ~1.0 mg/mL
• technika/technikák: ELISA: 0.1-0.2 μg/mL using 2.5 μg/mL Human IgM myeloma for coating; immunoblotting: suitable
• izotípus: IgG2b
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified

Leírás

Általános leírás

Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgM protein.

Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocyte membranes. IgM is suggested to act as the first line of defense during microbial infections. IgM has a pentameric structure, in which monomers are linking together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation.

Egyediség

Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal specifically recognizes m chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, k, l, and g chains.

Immunogén

Purified human IgM

Alkalmazás

The antibody is recommended to use in various immunological techniques, including ELISA and Immunoblot.

Biokémiai/fiziológiai hatások

Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.

Egyéb megjegyzések

This product is for R&D use only, not for drug, household, or other uses.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable