S8320

SigmaMarker^™

high range, mol wt 36,000-200,000 Da

• MDL-szám: MFCD00165844
• UNSPSC kód: 12352200

Tulajdonságok

• Forma: lyophilized powder
• használat: sufficient for 10-20 large gels (16 × 18 cm); sufficient for 20-30 mini-gels (10 × 10 cm); vial sufficient for 50-100 PhastGels
• molekulatömeg: 36,000-200,000 Da
• tárolási hőmérséklet: 2-8°C

Leírás

Alkalmazás

SigmaMarkers are designed for use with Laemmli SDS-PAGE, the PhastSystem (Pharmacia) and Schagger and von Jagow SDS-PAGE (Tricine). SigmaMarkers cover the range of molecular weights common to most proteins or their subunits. The wide, high and low range markers are lyophilized with sample buffer so that they are ready-to-use after reconstitution with deionized water. The markers are formulated to yield a distribution of well-defined bands of approximately equal intensity after electrophoresis and Coomassie blue staining.

Egyéb megjegyzések

Protein (mol. wt.)
Glyceraldehyde-3-phosphate dehydrogenase, rabbit muscle (36,000)
Ovalbumin, chicken egg (45,000)
Glutamic dehydrogenase, bovine liver (55,000)
Albumin, bovine serum (66,000)
Phosphorylase B, rabbit muscle (97,000)
β-Galactosidase, E. coli (116,000)
Myosin, porcine heart (200,000)

Will yield bands for the following seven proteins from the SigmaMarker list with Coomassie Blue staining.

Feloldás

Reconstitution with 100 μl of deionized water results in a solution containing 2-3.5 mg of protein per ml of 62 mM Tris-HCl, pH 6.8, 1 mM EDTA, 4% sucrose, 10 mM TCEP, 2% SDS, and 0.01% bromophenol blue.

Jogi információk

SigmaMarker is a trademark of Sigma-Aldrich Co. LLC

Biztonsági adatok

• Piktogramok: GHS08
• Figyelmeztetés: Danger
• Figyelmeztető mondatok: H334
• Óvintézkedésre vonatkozó mondatok: P261 - P284 - P501
• Veszélyességi osztályok: Resp. Sens. 1
• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, type N95 (US)