R4779

Anti-Rab7 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Szinonimák: Anti-Ras-Related Protein Rab7
• MDL-szám: MFCD08705509
• UNSPSC kód: 12352203
• NACRES: NA.46

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen ~23 kDa
• faj reaktivitás: mouse, human, rat
• technika/technikák: indirect immunofluorescence: 5-10 μg/mL using rat NRK and mouse NIH-3T3 cells fixed and permeabilized with 3% paraformaldehyde followed by 0.4% saponin; western blot: 2.5-5.0 μg/mL using extract of human A431 cells
• UniProt elérési szám: P51149
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... RAB7A(7879) ; mouse ... Rab7(19349) ; rat ... Rab7a(29448)

Leírás

Általános leírás

Rab7 is a member of the Rab family of small guanosine triphosphatases (GTPases). Rab proteins contain conserved regions involved in guanine nucleotide binding, and hypervariable COOH-terminal domains with a cysteine motif, implicated in subcellular targeting. Each Rab protein shows a characteristic subcellular distribution. The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

Ras-related protein Rab-7 (Rab7) is a member of the Rab family of small guanosine triphosphatases (GTPases).

Egyediség

Anti- Rab7 recognizes human, mouse and rat Rab7.

Immunogén

Synthetic peptide corresponding to amino acid residues of human Rab7 with C-terminal added cysteine, conjugated to KLH.

Alkalmazás

Anti-Rab7 antibody can be used in indirect immunofluorescence and immunoblotting.

Anti-Rab7 antibody produced in rabbit has been used in confocal microscopy.

Biokémiai/fiziológiai hatások

Rab family of small guanosine triphosphatases (GTPases) are central regulators of membrane trafficking between the different subcellular compartments of the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP-bound inactive and GTP-bound active states. Conversion from one state to the other is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment. Through their effector proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion. Rab7 is also involved in the maturation of late autophagic vacuoles. Among Rab7 effectors are RILP (Rab-interacting lysosome protein) that controls late endosomal and lysosomal transport by mediating the recruitment of dynein/dynactin motors, Rabring7 (Rab7- interacting ring finger protein),(10) and the hVPS34/p150 complex. Anti-Rab7 may be used as a marker for late endosomes.

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Tárolás és stabilitás

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• WGK: WGK 3
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable