Összes fotó(1)
Fontos dokumentumok
R4018
Ribonucleic acid, transfer, phenylalanine specific from brewer′s yeast
Szinonimák:
Transfer RNA, Yeast Phe tRNA, tRNA
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
Javasolt termékek
grade
for molecular biology
Forma
lyophilized powder
molekulatömeg
25 kDa
oldhatóság
water: 2 mg/mL
tárolási hőmérséklet
2-8°C
Általános leírás
Transfer RNA is isolated from brewer′s yeast. This transfer RNA is specific for transfer of the amino acid phenylalanine.
The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.
The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.
This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.
The phenylalanine acceptor activity of 100% pure tRNA is 1,800 pmoles per A260 unit. Comparison of this number to lot-specific analysis may be used as an indication of product purity. Methods to further purify tRNA have been published.
The assay for this product is based on a published method. The activity is measured using a crude preparation of phenylalanyl transfer ribonucleic acid synthetase. The phenylalanine specific tRNA is used as a substrate for the enzyme in the assay.
This product is not suitable for use as carrier tRNA to aid in the ethanol precipitation of very small amounts of DNA. Product Nos. R 8508 and R 5636 are recommended for use as carrier tRNA for DNA purification and precipitation.
Alkalmazás
Suitable for use:
- As a tRNA standard
- As a substrate in tRNA synthatase assays
- As a substrate in other reactions involving tRNAs
Komponensek
tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.
Elkészítési megjegyzés
Prepared by method of Wimmer, E., et al., Biochemistry, 7, 2623 (1968).
kapcsolódó termék
Product No.
Leírás
Árazás
Tárolási osztály kódja
13 - Non Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
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Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.
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