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Merck

R7876

Sigma-Aldrich

Ribonucleic acid, transfer from wheat germ

Type V, 15-19 units/mg solid

Szinonimák:

Transfer RNA, tRNA

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

CAS-szám:
MDL-szám:
UNSPSC kód:
12352106
NACRES:
NA.55

grade

for molecular biology

Minőségi szint

típus

Type V

form

lyophilized powder

specifikus aktivitás

15-19 units/mg solid

tárolási körülmény

desiccated

oldhatóság

H2O: ~2 mg/mL, clear to hazy

tárolási hőmérséklet

2-8°C

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Általános leírás

Wheat germ tRNA contains many different specific tRNA molecules.

Alkalmazás

Crude transfer RNA (tRNA), purified from wheat germ, contains many specific tRNA molecules that can be separated by different processes. Experiments for detailed investigation of structure and function using in vitro translation systems often require highly purified tRNA, which can be achieved by combinations of chromatographic separations and reversible biochemical modification of the tRNA. Wheat germ tRNA has been used for the purification of RNAse Z (tRNA 3′- processing endonucleases) from wheat germ. The purification included six steps with the most efficient one using an affinity column made of wheat germ tRNA, which the RNAse Z bound tightly.
Suitable for use as a carrier in nucleic acid purification and precipitation.

Komponensek

tRNA is provided as a lyophilized powder that is soluble in water at a concentration of 2 mg/ml.

Egység definíció

One unit will yield an absorbance of 1.0 at 260 nm in 1.0 mL of water (1 cm light path).

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

Az ügyfelek ezeket is megtekintették

Spatial and temporal expression of the zebrafish genome by large-scale in situ hybridization screening.
Bernard Thisse et al.
Methods in cell biology, 77, 505-519 (2004-12-18)
Christèle Maison et al.
Nature genetics, 30(3), 329-334 (2002-02-19)
Post-translational modification of histone tails is thought to modulate higher-order chromatin structure. Combinations of modifications including acetylation, phosphorylation and methylation have been proposed to provide marks recognized by specific proteins. This is exemplified, in both mammalian cells and fission yeast
Yana P Blokhina et al.
PLoS genetics, 15(1), e1007730-e1007730 (2019-01-18)
Meiosis is a cellular program that generates haploid gametes for sexual reproduction. While chromosome events that contribute to reducing ploidy (homologous chromosome pairing, synapsis, and recombination) are well conserved, their execution varies across species and even between sexes of the
Fu-Jung Lin et al.
PLoS genetics, 9(5), e1003516-e1003516 (2013-05-30)
Mouse Ikbkap gene encodes IKAP--one of the core subunits of Elongator--and is thought to be involved in transcription. However, the biological function of IKAP, particularly within the context of an animal model, remains poorly characterized. We used a loss-of-function approach
Florian Eggenhofer et al.
Nucleic acids research, 41(Web Server issue), W499-W503 (2013-05-04)
A standard method for the identification of novel non-coding RNAs is homology search by covariance models. Covariance models are constructed for specific RNA families with common sequence and structure (e.g. transfer RNAs). Currently, there are models for 2208 families available

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