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PROTSIL2

Sigma-Aldrich

ProteoSilver™ Plus Silver Stain Kit

Szinonimák:

protein stain, silver stain

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)

About This Item

UNSPSC kód:
41105322
NACRES:
NA.32

használat

 kit sufficient for 25 mini-gels (10 × 10 cm)

Minőségi szint

200

technika/technikák

protein staining: suitable

tárolási hőmérséklet

20-25°C

Általános leírás

Conveniently packaged, extremely stable, and highly sensitive, ProteoSilver™ Plus Silver Stain Kit is an ideal product for any protein scientist. Similar to ProteoSilver (PROTSIL1), this kit contains prepared solutions of silver staining reagents along with detailed instructions to achieve optimal results. With a detection limit of 0.1 ng/mm2 of protein (BSA) and an extremely low background, ProteoSilver Plus leads to superior detection of low abundance proteins.
ProteoSilver Plus is also MALDI-MS compatible and does not contain glutaraldehyde, which crosslinks lysine residues, resulting in inaccurate spectra. ProteoSilver Plus Silver Stain Kit contains two additional reagents for destaining an excised protein spot, for those wishing to perform further characterization through tryptic digestion and MALDI-MS analysis.

Alkalmazás

ProteoSilver Silver Stain Kit has been used for staining of the gels for visualizing proteins. It has also been used for destaining of the protein of interest using destainer provided in the kit for mass spectroscopic analysis.

Tulajdonságok és előnyök

  • Premixed and preweighed solutions reduce time and cost of purchasing and preparing individual components
  • Optimized protocols help establish conditions for best results
  • High sensitivity and low background ensure very low abundance proteins can be detected and resolved from other proteins
  • MALDI Compatible - Protein spots of interest can be further characterized by mass spectrometry
  • Room Temperature Stability allows easy and convenient storage

Jogi információk

ProteoSilver is a trademark of Sigma-Aldrich Co. LLC

Figyelmeztetés

Danger

Veszélyességi osztályok

Acute Tox. 2 Inhalation - Acute Tox. 3 Dermal - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1B - Eye Dam. 1 - Met. Corr. 1 - Muta. 2 - Repr. 1B - Skin Corr. 1A - Skin Sens. 1 - STOT SE 2 - STOT SE 3

Célzott szervek

Eyes,Central nervous system, Respiratory system

Tárolási osztály kódja

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Lobbanási pont (F)

149.0 °F - closed cup

Lobbanási pont (C)

65 °C - closed cup

Válasszon a legfrissebb verziók közül:

Analitikai tanúsítványok (COA)

Lot/Batch Number
0000368096
0000368096
SLCP7180
SLCL8105
SLCJ4208

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Dokumentumtár megtekintése

Effect of biomolecules from human renal matrix of calcium oxalate monohydrate (CaOx) stones on in vitro calcium phosphate crystallization.
Pathak P, et al.
International Brazilian Journal of Urology : Official Journal of the Brazilian Society of Urology, 36, 621-628 (2010)
K P Aggarwal et al.
International braz j urol : official journal of the Brazilian Society of Urology, 39(1), 128-136 (2013-03-16)
Proteins constitute a major portion of the organic matrix of human calcium oxalate (CaOx) renal stones and the matrix is considered to be important in stone formation and growth. The present study evaluates the effect of these proteins on oxalate
Kanu Priya Aggarwal et al.
PloS one, 8(7), e69916-e69916 (2013-07-31)
The increasing number of patients suffering from urolithiasis represents one of the major challenges which nephrologists face worldwide today. For enhancing therapeutic outcomes of this disease, the pathogenic basis for the formation of renal stones is the need of hour.
Peeping into human renal calcium oxalate stone matrix: characterization of novel proteins involved in the intricate mechanism of urolithiasis.
Aggarwal KP, et al.
PLoS ONE, 8, e69916-e69916 (2013)
Slovénie Pyndiah et al.
Molecular & cellular proteomics : MCP, 6(2), 193-206 (2006-11-10)
The study of protein interactions constitutes an important domain to understand the physiology and pathogenesis of microorganisms. The two-dimensional blue native/SDS-PAGE was initially reported to analyze membrane protein complexes. In this study, both cytoplasmic and membrane complexes of a bacterium
View All Related Papers

Cikkek

Protein Staining Reagents Selection Guide

Sigma offers EZBlue™ and ProteoSilver™ reagents for protein visualization, suitable for proteomics and traditional PAGE formats.

Tudóscsoportunk valamennyi kutatási területen rendelkezik tapasztalattal, beleértve az élettudományt, az anyagtudományt, a kémiai szintézist, a kromatográfiát, az analitikát és még sok más területet.

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