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Merck
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Fontos dokumentumok

P8045

Sigma-Aldrich

Protein G-Biotin from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

Szinonimák:

Biotinylated Protein G, Protein G Biotin, Protein G-Biotin

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

MDL-szám:
UNSPSC kód:
12352203
NACRES:
NA.46

rekombináns

expressed in unspecified host

Minőségi szint

konjugátum

biotin conjugate

Forma

lyophilized powder

összetétel

Protein, ~90% biuret

a címkézés mértéke

2-4 mol d-biotin per mol protein

technika/technikák

direct ELISA: 1:70,000 using human IgG

tárolási hőmérséklet

2-8°C

Általános leírás

Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.
Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.

Egyediség

Binds IgG only from most mammalian species except cat; binds chicken IgG.

Alkalmazás

Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.
Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.

Kiszerelés

Package size based on protein content

Egyéb megjegyzések

Truncated - lacks albumin, Fab and membrane binding sites.

Elkészítési megjegyzés

Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Tárolási osztály kódja

11 - Combustible Solids

WGK

WGK 3

Lobbanási pont (F)

Not applicable

Lobbanási pont (C)

Not applicable

Egyéni védőeszköz

Eyeshields, Gloves, type N95 (US)


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Analitikai tanúsítványok (COA)

Lot/Batch Number

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Pierre-Henri Puech et al.
PloS one, 6(7), e22344-e22344 (2011-07-30)
The rupture forces and adhesion frequencies of single recognition complexes between an affinity selected peptide/MHC complex and a TCR at a murine hybridoma surface were measured using Atomic Force Microscopy. When the CD8 coreceptor is absent, the adhesion frequency depends
Ayumi Taniguchi et al.
Journal of nutritional science and vitaminology, 53(6), 457-463 (2008-01-19)
It is well known that biotin deficiency causes morphological anomalies in hatchlings of fowl. An abundance of biotin in the yolk, therefore, is greatly required for maintaining reproductive functions. Although there is growing evidence for the molecular significance of the
Principles of Bacterial Detection: Biosensors, Recognition Receptors and Microsystems: Biosensors, Recognition Receptors, and Microsystem, 21(7), 360-360 (2008)
Electrochemical biosensing based on universal affinity biocomposite platforms
Zacco E, et al.
Biosensors And Bioelectronics, 21(7), 1291-1301 (2006)
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Microbiology spectrum, 10(3), e0236921-e0236921 (2022-05-24)
Human herpesvirus-6 (HHV-6) contains two genes (U12 and U51) that encode putative homologues of human G-protein-coupled receptors like CCR1, CCR3, and CCR5. It has been shown that these viral proteins can be expressed on the surface of epithelial and some

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