Összes fotó(1)
Fontos dokumentumok
NA0150
GenElute™ HP Plasmid Miniprep Kit
sufficient for 70 preparations
Szinonimák:
GenElute™ HP Plasmid Kit, HP miniprep kit, fast miniprep kit, fast plasmid isolation kit, plasmid extraction kit, plasmid isolation kit, plasmid purification kit, ultrafast plasmid isolation kit, Gen Elute
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
UNSPSC kód:
41105501
NACRES:
NA.52
Javasolt termékek
használat
sufficient for 70 preparations
Minőségi szint
környezetbarátabb alternatív termék tulajdonságai
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
technika/technikák
DNA extraction: suitable
környezetbarátabb alternatív kategória
tárolási hőmérséklet
15-25°C
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Általános leírás
An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the plasmid DNA onto silica in the presence of high salts. Contaminants are then removed by a vacuum or spin wash steps. Finally, the bound plasmid DNA is eluted in water or Tris-EDTA buffer.
The GenElute™ HP Plasmid Miniprep Kit offers an ultrafast and efficient solution for plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology and the convenience of spin or vacuum format,up to 25 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimum volume of culture to use depend on the plasmid and the culture medium.
The GenElute™ HP Plasmid Miniprep Kit offers an ultrafast and efficient solution for plasmid preparation from E. coli cultures. This kit combines silica-based membrane technology and the convenience of spin or vacuum format,up to 25 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimum volume of culture to use depend on the plasmid and the culture medium.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.
Alkalmazás
The recovered plasmid DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transcription, and sequencing.
Tulajdonságok és előnyök
- Purified plasmid DNA in less than 30 minutes
- Up to 25 μg of high-copy plasmid DNA
- Flexibility of a vacuum or spin format
- No phenol/chloroform extraction or alcohol precipitation required
Jogi információk
GenElute is a trademark of Sigma-Aldrich Co. LLC
kapcsolódó termék
Product No.
Leírás
Árazás
Figyelmeztetés
Danger
Figyelmeztető mondatok
Óvintézkedésre vonatkozó mondatok
Veszélyességi osztályok
Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Célzott szervek
Central nervous system
Tárolási osztály kódja
3 - Flammable liquids
Lobbanási pont (F)
77.0 °F
Lobbanási pont (C)
25 °C
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