LYSC9000

Lys-c (lysyl-endopeptidase), Active

from Achromobacter lyticus, recombinant, expressed in E. coli (206-473aa), tag-free, solution

• Szinonimák: Lys-c (lysyl-endopeptidase), Active, API, Lysyl endopeptidase, Protease I
• Enzyme Commission szám: 3.4.21.50 (BRENDA, IUBMB)
• UNSPSC kód: 12352204
• NACRES: NA.54

Tulajdonságok

• biológiai forrás: Achromobacter lyticus
• Minőségi szint: 200
• rekombináns: expressed in E. coli (206-473aa)
• grade: Proteomics Grade
• form: ready-to-use solution
• koncentráció: 1 mg/mL
• optimális pH: 8.5
• pH-tartomány: 9.0-9.5(catalytic)
• UniProt elérési szám: P15636
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C

Leírás

Általános leírás

Recombinant tag-free Achromobacter lyticus Lys-c (lysyl-endopeptidase) (206-473aa) was expressed in E.coli cells.

Overview
Lysyl-endopeptidase (Lys-c) was isolated from the Gram-negative soil bacterium Achromobacter lyticus by Msaki et al. The protein hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues making it an important tool for enzymatic protein sequencing and Lys-X compound synthesis.

Egyediség

This enzyme hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine and S-aminoethylcysteine residues, at a catalytic pH range of 9.0-9.5, catalytic temperature range of 30-37 °C.

Alkalmazás

The enzyme functions optimally between 30-37 °C and suffers from degradation when subjected to temperatures above 50 °C. Lysyl-endopeptidase retains complete activity after incubation in 4M urea or in 0.1% SDS solution for up to 6 hours at 30 °C.

Kiszerelés

1mg/ml in Plastic

Elkészítési megjegyzés

Catalytic pH range 9.0-9.5. Catalytic temperature range 30-37 °C.

Tárolás és stabilitás

Store product at -20°C for up to one year. Avoid freeze/thaw cycles.

Egyéb megjegyzések

For R&D only.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: WGK 1
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable