ENDOARGS-RO

Endoproteinase Asp-N Sequencing Grade

from Pseudomonas fragi

• Szinonimák: protease
• Enzyme Commission szám: 3.4.24.33 (BRENDA, IUBMB)
• UNSPSC kód: 12352204

Tulajdonságok

• Teszt: ≥90% (SDS-PAGE)
• form: lyophilized
• specifikus aktivitás: ≥20000 units/mg protein
• molekulatömeg: 27 kDa
• kiszerelés: pkg of 2 μg (11420488001); pkg of 3 × 2 μg (11054589001)
• gyártó/kereskedő neve: Roche
• optimális pH: 7.0-8.0
• pH-tartomány: 6.5-9.0
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

The wild type of Pseudomonas fragi segregates a metalloprotease, which cleaves peptide bonds N-terminally at small hydrophilic amino acids. A mutant of this strain produces the Endoproteinase Asp-N Sequencing Grade, which is isolated as a highly purified and specific protease.

Egyediség

Endoproteinase Asp-N Sequencing Grade is a metalloprotease. In phosphate, acetate, or Tris buffers at pH 6.0–8.5, it specifically cleaves peptide bonds N-terminally at aspartic and cysteic acid. If cysteine is reduced or alkylated, only -↓-Asp-X is cleaved.
The specificity of Endoproteinase Asp-N Sequencing Grade is verified with glucagon as a substrate. High concentrations of Endoproteinase Asp-N Sequencing Grade (1 part by weight enzyme with 10 parts by weight glucagon) are incubated to detect traces of impurities like contaminating proteases.
Heat inactivation: The protease is stopped by boiling for 5 minutes.

Alkalmazás

Use Endoproteinase Asp-N Sequencing Grade for protein structure analysis and sequence analysis.

Elkészítési megjegyzés

Working concentration: 2 μg /50 μl double-dist. water
One vial (= 2 μg enzyme) is dissolved in 50 μl double-dist. water resulting in a buffer concentration of 10 mM Tris-HCl pH 7.5.
Working solution: Recommended solvent is double-distilled water.
Storage conditions (working solution): -15 to -25 °C
A solution in double-dist. water is stable for 1 week at maximum at 2 to 8 °C and for about 1 month at -15 to -25 °C; repeated thawing should be avoided.

Feloldás

Lyophilized Endoproteinase Asp-N sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 10 mM Tris-HCl, pH 7.5. In order to avoid autolysis the incubation temperature should not exceed 37 °C.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 1
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash