ENDOLYSS-RO

Endoproteinase Lys-C Sequencing Grade

from Lysobacter enzymogenes

• Szinonimák: lys-c, lysyl endopeptidase, protease
• Enzyme Commission szám: 3.4.21.50 (BRENDA, IUBMB)
• UNSPSC kód: 12352204

Tulajdonságok

• biológiai forrás: bacterial (Lysobacter enzymogenes)
• grade: protein sequencing grade
• form: lyophilized
• specifikus aktivitás: ≥200 units/mg protein (at 25 °C, with Chromozym PL)
• molekulatömeg: 33 kDa by SDS-PAGE (reducing)
• tisztítva: electrophoresis
• kiszerelés: pkg of 3 × 5 μg (11047825001); pkg of 5 μg (11420429001)
• gyártó/kereskedő neve: Roche
• tárolási körülmény: (Keep container tightly closed in a dry and well-ventilated place.)
• technika/technikák: activity assay: suitable
• szín: white
• optimális pH: 8.5-8.8
• pH-tartomány: 5-12
• oldhatóság: water: soluble
• alkalmasság: suitable for enzyme test
• UniProt elérési szám: Q7M135
• tárolási hőmérséklet: 2-8°C

Leírás

Általános leírás

Endoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease. Endoproteinase Lys-C from Lysobacter enzymogenes is a member of the peptidase S1 family. It is mostly used for the initial fragmentation of polypeptide chains in protein sequence analysis.

Egyediség

This enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0).
Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA.

Alkalmazás

Use Endoproteinase Lys-C Sequencing Grade  for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.

Elkészítési megjegyzés

Storage conditions (working solution): +2 to +8 °C

Feloldás

Lyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C.
Stable in 4 M urea.

Egyéb megjegyzések

For life science research only. Not for use in diagnostic procedures.

Biztonsági adatok

• Tárolási osztály kódja: 11 - Combustible Solids
• WGK: WGK 2
• Lobbanási pont (F): does not flash
• Lobbanási pont (C): does not flash