H9539

Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Szinonimák: Anti-Amphoterin, Anti-High mobility group box 1, Anti-High mobility group protein 1
• MDL-szám: MFCD02263062
• UNSPSC kód: 12352203
• NACRES: NA.41

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen 25 kDa
• faj reaktivitás: rat, mouse, human
• kiszerelés: antibody small pack of 25 μL
• fejlettebb validálás: independent; Learn more about Antibody Enhanced Validation
• technika/technikák: immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates.; indirect immunofluorescence: suitable; western blot: 0.5-1 μg/mL using 3T3 or PC12 cell lysates.
• UniProt elérési szám: P09429
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... HMGB1(3146) ; mouse ... Hmgb1(15289) ; rat ... Hmgb1(25459)

Leírás

Általános leírás

The High Mobility Group (HMG1) is secreted by activated monocytes and macrophages. The gene is mapped to human chromosome 13q12.

Immunogén

synthetic peptide corresponding to amino acid 165-180 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat.

Alkalmazás

Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit has been used in ELISA and immunofluorescence.

Biokémiai/fiziológiai hatások

In the nucleus, HMGB1 (high-mobility group protein B1) works as a nucleosome stabilizer and transcriptional regulator. It also exhibits chaperone-like activity and suppresses aggregation of polyglutamine. It has a role in inflammation and autophagy. HMGB1 can interact with toll-like receptor (TLR) 2 and 4, interleukin-1β, chemokine (C-X-C motif) ligand 12 (CXCL12) and pathogen-associated molecular pattern (PAMP) molecules. It also works as a damage-associated molecular pattern molecule (DAMP).

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Tárolás és stabilitás

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots.
Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Egyéb megjegyzések

In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 12 - Non Combustible Liquids
• WGK: nwg
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)