Ugrás a tartalomra
Merck

GL0010

Sigma-Aldrich

Golgi Isolation Kit

sufficient for 50 g (tissue)

Szinonimák:

Golgi Kit, Isolation Kit for Golgi

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12352200
NACRES:
NA.32

használat

sufficient for 50 g (tissue)

Minőségi szint

technika/technikák

fractionation: suitable

kiszállítva

wet ice

tárolási hőmérséklet

2-8°C

Általános leírás

The Golgi Isolation Kit provides a method for isolating Golgi membranes from mammalian soft tissues by discontinuous density gradient. The degree of Golgi enrichment can be determined by assaying the acitivty of UDP-galactosyl transferase or by immunodetection of Golgi specific marker proteins like B-COP or GM130 using appropriate antibodies (Cat. No. G6160 and G7295, respectively). Separation from other organelles can be measured using the appropriate marker detection kits (Cat. No. CS0780, CYTOCOX1, CY0100 and CAT100).

Alkalmazás

Golgi Isolation Kit may be used for the isolation of Golgi membranes from mammalian soft tissues by discontinuous density gradient.

Analízis megjegyzés

The Golgi Isolation kit was optimized using rat liver and tested on rat kidney, spleen, and heart.

Kit Components Also Available Separately

Product No.
Leírás
Biztonsági adatlap

  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 5 mLBiztonsági adatlap

Tárolási osztály kódja

10 - Combustible liquids

WGK

WGK 3


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Analitikai tanúsítványok (COA)

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Dokumentumtár megtekintése

V J Allan et al.
The Journal of cell biology, 113(2), 347-359 (1991-04-01)
When higher eukaryotic cells enter mitosis, membrane organization changes dramatically and traffic between membrane compartments is inhibited. Since membrane transport along microtubules is involved in secretion, endocytosis, and the positioning of organelles during interphase, we have explored whether the mitotic
E R Sjoberg et al.
The Journal of biological chemistry, 268(14), 10185-10196 (1993-05-15)
The melanoma-associated disialogangliosides 9(7)-O-acetyl-GD3 and 9(7)-O-acetyl-GD2 have been structurally well characterized. However, the compartmentalization and sequence of action of the biosynthetic activities responsible for synthesizing these molecules remain obscure. Here, we have studied the spatial and temporal interrelationships among the
Min Zhang et al.
Methods in molecular biology (Clifton, N.J.), 1880, 135-148 (2019-01-06)
Autophagy is a catabolic pathway for bulk turnover of cytoplasmic components through the lysosome. Completion of autophagy requires a sophisticated membrane remodeling process. The early steps involve autophagic membrane precursor generation from the intracellular membranes. The intricate protein-membrane interactions underlying
Julien Villeneuve et al.
The Journal of cell biology, 217(2), 649-665 (2017-12-08)
An appreciation of the functional properties of the cytoplasmic fatty acid binding protein 4 (FABP4) has advanced with the recent demonstration that an extracellular form secreted by adipocytes regulates a wide range of physiological functions. Little, however, is known about
A Surroca et al.
The Journal of membrane biology, 177(3), 243-249 (2000-10-03)
We investigated the direct effect of inositol 1,4,5-trisphosphate (IP(3)) and ryanodine receptor agonists on Ca(2+) release from vesicles of a rat liver Golgi apparatus (GA) enriched fraction, which were actively loaded with (45)Ca(2+). Results in GA were compared with those

Cikkek

Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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