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Merck

GE17-0780-01

Protein A-Sepharose CL-4B

Cytiva 17-0780-01, pack of 1.5 g

Szinonimák:

Protein A Sepharose, Sepharose CL-4B

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
41106500
NACRES:
NA.56

ligand

native protein A

kiszerelés

pack of 1.5 g

gyártó/kereskedő neve

Cytiva 17-0780-01

tárolási körülmény

(20% Ehtanol)

matrix

4% cross-linked agarose

részecskeméret

45-165 μm

átlagos átmérő

90 μm

cleaning

2-10

working range

3-9

kapacitás

~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

tárolási hőmérséklet

2-8°C

Related Categories

Általános leírás

Protein A Sepharose CL-4B is a classic, well documented medium for immunoglobin purification and fractionation, and is suitable for immunoprecipitation procedures.

Protein A binds to the Fc region of immunoglobulins through interactions with the heavy chain. The binding of protein A has been well documented for IgG from a variety of mammalian species and for some IgM and IgA as well. Protein A Sepharose CL-4B has been used as a powerful tool to isolate and purify classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Since only the Fc region is involved in binding, the Fab region is available for binding antigen.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Tulajdonságok és előnyök

  • Well documented for immunoglobulin purification and fractionation
  • Strong binding to Fc region of IgG
  • High capacity - 20 mg human IgG/mL.
  • Suited for immunoprecipitation procedures

Tárolás és stabilitás

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analízis megjegyzés

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Jogi információk

Sepharose is a trademark of Cytiva

Tárolási osztály kódja

13 - Non Combustible Solids


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

Már rendelkezik ezzel a termékkel?

Az Ön által nemrégiben megvásárolt termékekre vonatkozó dokumentumokat a Dokumentumtárban találja.

Dokumentumtár megtekintése

Daniel L Galvan et al.
The Journal of clinical investigation, 129(7), 2807-2823 (2019-05-08)
Phosphorylation of Dynamin-related protein1 (Drp1) represents an important regulatory mechanism for mitochondrial fission. Here we established the role of Drp1 Serine 600 (S600) phosphorylation on mitochondrial fission in vivo, and assessed the functional consequences of targeted elimination of the Drp1S600
Anna Kaisa Kylmä et al.
British journal of cancer, 119(1), 89-95 (2018-06-23)
An opportunistic oral pathogen, Treponema denticola (Td), has been linked to orodigestive carcinogenesis, but its role in oropharyngeal squamous cell carcinoma (OPSCC) has remained open. We evaluated the presence of Td chymotrypsin-like protease (Td-CTLP) in a series of 201 unselected
Vishal Kothari et al.
PloS one, 17(5), e0266472-e0266472 (2022-05-07)
Fetuin-A (Fet-A) is a liver-secreted phosphorylated protein, known to impair insulin signaling, which has been shown to be associated with obesity, insulin resistance, and incident diabetes. Fet-A interacts with the insulin-stimulated insulin receptor (IR) and inhibits IR tyrosine kinase activity
Anahita Nejatfard et al.
Cell reports, 37(3), 109840-109840 (2021-10-24)
Nearly one-third of proteins are initially targeted to the endoplasmic reticulum (ER) membrane, where they are correctly folded and then delivered to their final cellular destinations. To prevent the accumulation of misfolded membrane proteins, ER-associated degradation (ERAD) moves these clients
Shelby M Knoche et al.
Immunologic research, 70(3), 371-391 (2022-03-19)
Pancreatic cancer is one of the deadliest neoplasms, with a dismal 5-year survival rate of only 10%. The ability of pancreatic cancer cells to evade the immune system hinders an anti-tumor response and contributes to the poor survival rate. Downregulation

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