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Merck

GALS

Sigma-Aldrich

β-Galactosidase Reporter Gene Staining Kit

Szinonimák:

β-galactosidase, LacZ

Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez


About This Item

UNSPSC kód:
12161503
NACRES:
NA.32

használat

 kit sufficient for 100 tests (using a 3.5 cm dish)

Minőségi szint

technika/technikák

microbe id | staining: suitable

kiszállítva

dry ice

tárolási hőmérséklet

−20°C

Általános leírás

β-galactosidase (β-gal) catalyzes the conversion of X-gal to galactose and 5-bromo-4-chloro-3-hydroxyindole. The functionality of β-gal is indirectly measured by the blue precipitate of dimeric 5-bromo-4-chloro-3-hydroxyindole in the presence of potassium ferricyanide or ferrocyanide. The colorimetric detection methods also use other substrates of β-gal like O-nitrophenyl-β-D-galactopyranoside (ONPG). β-gal is a cost-effective, simple and standard reporter system. The Galactosidase Reproter Gene Staining Kit provides a method for determining the percentage of cells transfected with the plasmid expressing the β-galactosidase gene, LacZ. It can also be used to visualize the specific expression of the reporter gene in tissue sections by microscopy.

Alkalmazás

β-Galactosidase Reporter Gene Staining Kit has been used for histochemical staining of animal cells or tissues such as:
  • sagittal brain sections in histoenzymology
  • pancreas or colon sections in β-galactosidase reporter gene assay
  • mice E10.5 -11.5 embryo and embryo sections

Egyéb megjegyzések

The substrate used in this kit is X-Gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) which results in an indigo blue-colored staining of transfected cells and tissues. This test provides a method for determining the percentage of transfected cells that are expressing LacZ or for microscopically visualizing reporter gene expression in tissue sections.

kapcsolódó termék

Product No.
Leírás
Árazás

Figyelmeztetés

Danger

Veszélyességi osztályok

Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Flam. Liq. 3 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3

Célzott szervek

Respiratory system

Egyéb veszélyek

Tárolási osztály kódja

3 - Flammable liquids

Lobbanási pont (F)

132.8 °F

Lobbanási pont (C)

56 °C


Analitikai tanúsítványok (COA)

Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.

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Dokumentumtár megtekintése

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