G25150
Sephadex® G-25
Medium
Szinonimák:
Sephadex G-25 Medium, Sephadex® G-25 resins, Size exclusion resins
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(2)
About This Item
Javasolt termékek
Minőségi szint
technika/technikák
buffer exchange: suitable
mátrix aktív csoport
phase
duzzanat
1 g swells to 4-6 mL
gyöngyméret
50-150 μm
alkalmazás(ok)
life science and biopharma
kompatibilitás
Cytiva
InChI
1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
Nemzetközi kémiai azonosító kulcs
BCPVZFFJGCURNR-OCOFDJSDSA-N
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Általános leírás
Sephadex® G-25 Medium is a size exclusion chromatography or gel filtration resin that is created by crosslinking dextran with epichlorohydrin. The various types of Sephadex can differ in their degree of cross-linking, resulting in variances in their swelling capacity and molecular fractionation range. Sephadex G-25 is one option available among the different G-types of Sephadex. Sephadex G-25 belongs to a range of five G-types, catering to different molecule sizes. Specifically, G-10 is suitable for small molecules, while G-75 is designed to accommodate larger molecules. Sephadex G-25 provides industrial requirements by ensuring a dependable supply and offering extensive technical and regulatory assistance.
Alkalmazás
Fractionation Range (MW)
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000
Sephadex® G-25 has been used:
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000
Sephadex® G-25 has been used:
- in the desalting of protease
- in the column for the separation of radioiodinated oTP-1 from free iodine during the characterization of radioiodinated oTP-1 and receptor assay validation
Sephadex® G-25 has been used to separate the labelled proteins from unreacted dye N-hydroxysuccinimide (NHS) esters by gel permeation chromatography.
Biokémiai/fiziológiai hatások
Sephadex® G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex® G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.
Tulajdonságok és előnyök
- Rapid desalting, contaminant removal, and transfer to a new buffer in a single step.
- High recovery rate with minimal sample dilution.
- Offered in prepacked HiPrep Desalting and HiTrap desalting columns for convenient and fast desalting.
- Specifically designed as a BioProcess resin for industrial applications.
Jogi információk
Sephadex is a registered trademark of Cytiva
helyettesítve
Tárolási osztály kódja
11 - Combustible Solids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves, type N95 (US)
Analitikai tanúsítványok (COA)
Analitikai tanúsítványok (COA) keresése a termék sarzs-/tételszámának megadásával. A sarzs- és tételszámok a termék címkéjén találhatók, a „Lot” vagy „Batch” szavak után.
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