Összes fotó(1)
Fontos dokumentumok
D4764
Deoxyribonucleic acid from calf thymus
Genomic, unsheared
Szinonimák:
double-stranded template DNA, Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid
Bejelentkezésa Szervezeti és Szerződéses árazás megtekintéséhez
Összes fotó(1)
About This Item
CAS-szám:
EC-szám:
MDL-szám:
UNSPSC kód:
41106310
eCl@ss:
32160414
NACRES:
NA.52
Javasolt termékek
grade
for molecular biology
Minőségi szint
Forma
lyophilized powder
molekulatömeg
>20 kb
tárolási hőmérséklet
2-8°C
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Általános leírás
High quality double-stranded template DNA isolated from the thymus of male and female calves.
Egyediség
42% GC; Tm = 87 °C.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
% GC is the percentage of G/C base pairs. Tm is the temperature at the midpoint of the thermal denaturation profile.
Alkalmazás
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard for DNA quantification by fluorescent assay. It was used as reference to determine the DNA content in cartilage tissue digest samples.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.
Tulajdonságok és előnyök
• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA
• High quality template DNA
Elkészítési megjegyzés
Purified by anion exchange chromatography, dialyzed against a solution of 1 mM sodium chloride, 1 mM EDTA and 1 mM Trizma HCl, pH 7.5 and lyophilized at a concentration of ~2 A260 units per mL. ~20 A260 units per mg DNA.
Feloldás
Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
Tárolási osztály kódja
10 - Combustible liquids
WGK
WGK 3
Lobbanási pont (F)
Not applicable
Lobbanási pont (C)
Not applicable
Egyéni védőeszköz
Eyeshields, Gloves
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