D1319

Anti-DAP Kinase 1 antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• Szinonimák: Anti-DAPK, Anti-DAPK1, Anti-Death associated protein kinase 1
• UNSPSC kód: 12352203
• NACRES: NA.44

Tulajdonságok

• biológiai forrás: rabbit
• Minőségi szint: 200
• konjugátum: unconjugated
• antitest forma: affinity isolated antibody
• antitest terméktípus: primary antibodies
• klón: polyclonal
• Forma: buffered aqueous solution
• molekulatömeg: antigen ~160 kDa
• faj reaktivitás: mouse (predicted), human, rat (predicted)
• koncentráció: ~1 mg/mL
• technika/technikák: immunoprecipitation (IP): 2.5-5.0 μg using extracts of human HeLa cells; western blot: 1-2 μg/mL using whole extracts of human HeLa cells
• UniProt elérési szám: P53355
• kiszállítva: dry ice
• tárolási hőmérséklet: −20°C
• célzott transzláció utáni módosítás: unmodified
• Géninformáció: human ... DAPK1(1612) ; mouse ... Dapk1(69635) ; rat ... Dapk1(306722)

Leírás

Általános leírás

DAP-kinase 1 is a Ca²⁺/calmodulin dependent Ser/Thr kinase that associates with microfilaments. The protein is composed of a multidomain structure. It has a subdomain typical of serine/threonine kinases, a Ca²⁺/calmodulin regulatory domain, eight ankyrin repeats followed by two P-loop motifs and a typical death domain module. It contains two auto-inhibitory domains, one being Ca²⁺/calmodulin dependent.

Egyediség

Anti-DAPK1 antibody is specific for human DAPK1 (approx. 160 kDa). In immunoblotting, detection of the DAPK1 band is specifically inhibited with the immunizing peptide.

Immunogén

synthetic peptide corresponding to amino acids 2-13 of human DAP-kinase 1, conjugated to KLH via a C-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Alkalmazás

Anti-DAP Kinase 1 antibody is suitable for use in immunoprecipitation (2.5-5.0 μg using extracts of human HeLa cells) and western blot (1-2 μg/mL using whole extracts of human HeLa cells).

Anti-DAP Kinase 1 antibody produced in rabbit has been used in immunoprecipitation and western blotting.

Biokémiai/fiziológiai hatások

Death-associated protein kinase (DAPK) belongs to the serine/threonine kinase family that regulates apoptosis, growth factor signaling and also may be involved in modulating immune responses. DAPK1 mediates cellular functions such as membrane blebbing and autophagy and inhibits the activity of prolyl isomerase

Fizikai forma

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Jogi nyilatkozat

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biztonsági adatok

• Tárolási osztály kódja: 10 - Combustible liquids
• Lobbanási pont (F): Not applicable
• Lobbanási pont (C): Not applicable
• Egyéni védőeszköz: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)