KELLY Cell Line human

92110411, human brain, Neuronal

• Szinonimák: NB19 Cells, NB19-RIKEN Cells
• UNSPSC kód: 41106514

Tulajdonságok

• product name: KELLY Cell Line human, 92110411, neuroblastoma
• biológiai forrás: human brain
• növekedési mód: Adherent
• kariotípus: Not specified
• morfológia: Neuronal
• termékek: N-myc RNA or protein
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human neuroblastoma

Sejtvonal leírása

The cells possess a genomic amplification of the N-myc gene and express elevated levels of N-myc RNA or protein.

Alkalmazás

KELLY cell line has been used to study the role of polysialic acid (PSA) in controlling tumor cell growth and differentiation by interfering with neural cell adhesion molecule (NCAM) signaling. It has also been used to study the role of protein tyrosine phosphatase SHP1 in inhibiting Ret oncogene activity.

Táptalaj

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:5 to 1:20 i.e. seeding at 5x1,000-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Egyéb megjegyzések

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