BAE-1

88031149, bovine cardiovascular , Endothelial

• UNSPSC kód: 41106514

Tulajdonságok

• product name: BAE-1, 88031149
• biológiai forrás: bovine cardiovascular
• növekedési mód: Adherent
• kariotípus: 59 or 60
• morfológia: Endothelial
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Bovine aortic endothelium

Sejtvonal leírása

Derived from the aorta of a Friesian cow. Cells form "sprouts" after confluence. Infected with Bovine Viral Diarrhoea Virus (BVDV) which is a common contaminant of ruminants and is non-hazardous to humans. Secretes proteoglycans and collagen.

Táptalaj

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. There is a tendancy for the cells to grow fibroblast-like until they are confluent. Once confluent the endothelial morphology prevail

Egyéb megjegyzések

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