PANC-1 Cell Line human

87092802, human pancreas, Epithelial

• Szinonimák: PANC1 Cells, Panc-1-P Cells
• UNSPSC kód: 41106514
• eCl@ss: 32190102
• biológiai forrás: human pancreas
• növekedési mód: Adherent
• kariotípus: 2n = 46, hypertriploid
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• vonatkozó betegség(ek): cancer

Tulajdonságok

• Terméknév: PANC-1 Cell Line human, 87092802, from human pancreas
• biológiai forrás: human pancreas
• növekedési mód: Adherent
• kariotípus: 2n = 46, hypertriploid
• morfológia: Epithelial
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human Caucasian pancreas

Sejtvonal leírása

Established from a pancreatic carcinoma of ductal origin from a 56-year-old Caucasian male. Cells possess the type B phenotype for G6PD. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

Alkalmazás

Human PANC-1 cell line has been used to study the role of the intermediate filament keratin reorganization on the alignment and migration of cancer cells. It has also been used to study calcium-mediated actin reset (CaAR) in response to physiological changes.

DNS profil

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11
D5S818: 11,13
D7S820: 8,10
THO1: 7,8
TPOX: 8,11
vWA: 15

Táptalaj

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Egyéb megjegyzések

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