KG-1

86111306, human bone marrow, Myeloblast-like

• Szinonimák: KG1 Cells
• UNSPSC kód: 41106514

Tulajdonságok

• Terméknév: KG-1, 86111306
• biológiai forrás: human bone marrow
• növekedési mód: Suspension
• kariotípus: 2n = 46, diploid
• morfológia: Myeloblast-like
• termékek: Not specified
• receptorok: Not specified
• technika/technikák: cell culture | mammalian: suitable
• vonatkozó betegség(ek): cancer
• kiszállítva: dry ice
• tárolási hőmérséklet: −196°C

Leírás

Sejtvonal eredete

Human Caucasian bone marrow myelogenous leukaemia

Sejtvonal leírása

Derived from the bone marrow aspirate of a 59 year old male Caucasian with erythroleukemia that became acute myelogenous leukaemia. The cells form colonies in soft-agar in the presence of colony stimulating factor (CSF). KG-1 cells resemble acute myelogenous leukaemia showing considerable pleomorphism with a predominance of myeloblasts and promyelocytes. A small percentage of the cells are mature granulocytes with the occasional presence of macrophages and eosinophils. They do not express surface Ig or EBV antigens and lack specific markers for lymphocytic cells.

Alkalmazás

KG-1 has been used to extract total RNA and use them as templates to determine the 5′ end of promyelocytic leukemia zinc finger (PLZF) transcripts.

DNS profil

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7
D13S317: 11,12
D16S539: 10,11
D5S818: 13
D7S820: 8,10
THO1: 7,8
TPOX: 7,9
vWA: 14,19

Táptalaj

IDMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Szubkultúra rutin

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

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